Factors Affecting Ovum Pick-Up (OPU) in Friesian Cattle: A Review
DOI:
https://doi.org/10.14738/dafs.1404.12043Keywords:
Ovum pick-up, Friesian cattle, oocyte competence, embryo production, follicular dynamics, reproductive biotechnologyAbstract
Ovum pick-up (OPU) combined with in vitro embryo production (IVEP) has become an important reproductive biotechnology in dairy cattle breeding programs. The technique allows repeated recovery of oocytes from valuable donor animals, thereby accelerating genetic improvement and increasing reproductive efficiency. Friesian cattle, owing to their high milk-producing potential, are extensively utilized in OPU programs worldwide. However, the success of OPU depends on several biological, environmental, nutritional, hormonal, and technical factors that influence follicular development, oocyte quality, and embryo production. This review summarizes the major factors affecting OPU efficiency in Friesian cattle, including age, genetic background, nutrition, hormonal stimulation, follicular dynamics, heat stress, health status, operator expertise, and management practices. Understanding these factors is essential for optimizing oocyte recovery and improving the outcomes of assisted reproductive technologies in dairy herds.
References
Boni, R. (2012). Heat stress, a serious threat to reproductive efficiency in dairy cattle. Animal Frontiers, 2(1), 6–11.
Bó, G. A., Mapletoft, R. J., & Adams, G. P. (2018). Ovarian stimulation protocols in cattle. Theriogenology, 112, 3–16.
Chaubal, S. A., Ferre, L. B., Molina, J. A., Faber, D. C., Bols, P. E., Rezamand, P., Tian, X., & Yang, X. (2006). Hormonal treatments for increasing oocyte competence in cattle. Theriogenology, 65(1), 191–199.
De Roover, R., Bols, P. E., Genicot, G., Hanzen, C., & Dessy, F. (2008). Characterization of bovine follicular dynamics to improve OPU outcomes. Reproduction in Domestic Animals, 43, 81–85.
Garnsworthy, P. C., Sinclair, K. D., & Webb, R. (2008). Integration of physiological mechanisms regulating fertility in dairy cows. Animal, 2(8), 1144–1152.
Lonergan, P., Fair, T., Forde, N., & Rizos, D. (2016). Embryo development and quality in cattle. Animal Reproduction, 13(3), 264–272.
Merton, J. S., de Roos, A. P. W., Mullaart, E., de Ruigh, L., Kaal, L., Vos, P. L., & Dieleman, S. J. (2003). Factors affecting oocyte and embryo production in cattle. Theriogenology, 60(4), 651–658.
Pieterse, M. C., Kappen, K. A., Kruip, T. A. M., & Taverne, M. A. M. (1988). Aspiration of bovine oocytes during transvaginal ultrasound scanning. Theriogenology, 30(4), 751–762.
Pontes, J. H. F., Melo Sterza, F. A., Basso, A. C., Ferreira, C. R., Sanches, B. V., Rubin, K. C. P., & Seneda, M. M. (2011). Ovum pick-up, in vitro embryo production, and pregnancy rates in dairy cattle. Theriogenology, 75(5), 900–906.
Rizos, D., Ward, F., Duffy, P., Boland, M. P., & Lonergan, P. (2002). Consequences of bovine oocyte maturation conditions for embryo development. Theriogenology, 58(6), 1241–1251.
Santos, J. E. P., Cerri, R. L. A., & Sartori, R. (2008). Nutritional management and reproduction of dairy cattle. Theriogenology, 70(3), 409–419.
Sirard, M. A., Richard, F., Blondin, P., & Robert, C. (2006). Contribution of follicular environment to oocyte competence. Theriogenology, 65(1), 119–131.
Snijders, S. E. M., Dillon, P., O'Callaghan, D., & Boland, M. P. (2000). Effect of genetic merit and nutrition on embryo production in dairy cattle. Theriogenology, 53(4), 981–994.
Walsh, S. W., Williams, E. J., & Evans, A. C. O. (2011). A review of the causes of poor fertility in high milk-producing dairy cows. Animal Reproduction Science, 123(3–4), 127–138.
Watanabe, Y. F., Souza, A. H., Mingoti, R. D., Ferreira, R. M., Batista, E. O., Dayan, A., & Baruselli, P. S. (2017). Number of oocytes retrieved per donor during OPU and its effects on embryo production. Theriogenology, 87, 15–21.
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Copyright (c) 2026 Muhammad Ali Raza, Kissa Zahra Baloch, Laraib Zahra, Usman Ahmed, Salman Mehmood

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