Rethinking Structure; Challenges in the Development of Gluten-Free Pasta and Baked Goods: A Review
DOI:
https://doi.org/10.14738/tnc.1401.19861Abstract
The development of gluten-free products entails substantial technological and formulation challenges, primarily due to the difficulty of reproducing the structural, sensory, and nutritional attributes of gluten-containing analogues. The absence of gluten—responsible for the viscoelastic network that confers extensibility, gas retention, and structural integrity to wheat-based doughs—represents a major limitation in processing and product quality. To compensate for this deficiency, gluten-free formulations commonly incorporate optimized combinations of starches, hydrocolloids, and plant proteins to establish an alternative network. Starch gelatinization and the formation of a cohesive, viscous matrix by gums such as xanthan and guar contribute to dough integrity and rheological performance. Concurrently, the interactions between vegetable proteins and these polysaccharide components promote gas retention and stabilize the internal structure during fermentation and baking. This composite system partially replicates the viscoelastic behavior of gluten, thereby improving the texture, stability, and overall quality of gluten-free products.
References
Abdelgadir, H. A., Aboshora, W., & Babiker, E. E. (2022). Physicochemical and functional properties of starches with varying amylose-to-amylopectin ratios. International Journal of Biological Macromolecules, 209, 789–798. https://doi.org/10.1016/j.ijbiomac.2022.03.174.
Almeida, E. L., Chang, Y. K., & Steel, C. J. (2013). Application of texture analysis to elucidate mechanisms of texture changes in gluten-free bread. Food Science and Technology International. DOI: 10.1177/1082013212458627.
Baptista, N. T., Dessalles, R., Illner, A.-K., Ville, P., Ribet, L., Anton, P. M., & Durand-Dubief, M. (2024). Harnessing the power of resistant starch: A narrative review of its health impact and processing challenges. Frontiers in Nutrition, 11, 1369950. https://doi.org/10.3389/fnut.2024.1369950
Brasil, D. S. B., et al. (2015). Technological aspects and acceptance of gluten-free bread based on quinoa flour. Food Science and Technology International, 21(3), 188-199. DOI: 10.1177/1082013214536862.
Comettant-Rabanal, Raúl, Hidalgo Chávez, Davy William, Ascheri, José Luis Ramírez, Elías-Peñafiel, Carvalho, Carlos W Piler. Functionality of pre-cooked whole-grain corn, rice and sorghum flours for gluten-free bread, International Journal of Food Science and Technology, v.58, n.11, 2023, DOI: 10.1111/ijfs.16676
Chuqui-Paulino, F. J., Chávez, D. W. H., Ascheri, J. L. R., Mellinger, C. G., Vargas-Solorzano, J. W., & Carvalho, C. W. P. (2025). Impact of Whole Cereal–Pulse Flours on the Functionality and Antioxidant Properties of Gluten-Free Extruded Flours. Foods, 14(20), 3515. Scholar Publishing
Delcour, J. A., & Hoseney, R. C. (2020). Principles of cereal science and technology (4th ed.). AACC International Press.
Di Rosa, C., Fiore, A., & Padalino, L. (2023). Influence of high-amylose wheat flour on postprandial glycaemic response and technological properties of baked products. Foods, 12(14), 2719.
Fernandes, S. d. S., Vargas-Solórzano, J. W., Carvalho, C. W. P., & Ascheri, J. L. R. (2025). Innovative Gluten-Free Fusilli Noodle Formulation: Leveraging Extruded Japanese Rice and Chickpea Flours. Foods, 14(14), 2524. https://doi.org/10.3390/foods14142524.
Fundação do Câncer. (2019). Estudo sobre a prevalência de doença celíaca no Brasil. Disponível em: https://doi.org/10.1016/j.foodchem.2018.12.034.
Goesaert, H., Lagrain, B., & Delcour, J. A. (2021). The interplay of starch, gluten, and lipids in bread making. Cereal Chemistry, 98(3), 607–620. https://doi.org/10.1002/cche.10447.
Giuntini, E. B., Sardá, F. A. H., & de Menezes, E. W. (2022). The Effects of Soluble Dietary Fibers on Glycemic Response: An Overview and Futures Perspectives. Foods, 11(23), 3934.
Hoover, R. (2010). The impact of heat–moisture treatment on molecular structures and properties of starches isolated from different botanical sources. Critical Reviews in Food Science and Nutrition, 50(9), 835–847. https://doi.org/10.1080/10408390903001735.
Hoover, R., Ratnayake, W. S., & Noda, T. (2020). Physicochemical properties of starches from different botanical sources. Food Hydrocolloids, 108, 106024.
Horstmann, S. W., Lynch, K. M., & Arendt, E. K. (2017). Starch characteristics linked to gluten-free products: Their role in structure formation and textural quality. Comprehensive Reviews in Food Science and Food Safety, 16(5), 850–867. https://doi.org/10.1111/1541-4337.12279
Jane, J., Chen, J., & LeCorre, D. (2021). Structure, function, and applications of starches in food systems. Annual Review of Food Science and Technology, 12, 1–26. https://doi.org/10.1146/annurev-food-062420-123933.
Jebo, K. K., & Urga, K. (2024). Sensory characteristics and nutritional value of wheat-sorghum based bread enhanced with orange fleshed sweet potato. Cogent Food & Agriculture, 10(1). https://doi.org/10.1080/23311932.2024.2438403.
Kieffer, R., Wieser, H., & Koehler, P. (2019). Influence of glutenin polymerization on the viscoelastic properties of wheat dough. Journal of Cereal Science, 87, 34–42. https://doi.org/10.1016/j.jcs.2019.03.004.
Koehler, P., & Wieser, H. (2023). Wheat gluten proteins: Structure, properties, and role in baking. Springer Nature. https://doi.org/10.1007/978-3-031-13735-1.
Korkerd, S., Charoenrein, S., & Pradipasena, P. (2016). Influence of amylose and amylopectin on physicochemical properties of extruded starch. Journal of Food Engineering, 173, 1–8. https://doi.org/10.1016/j.jfoodeng.2015.10.034.
Lazou, A., & Krokida, M. (2010). Structural and textural characterization of corn and rice extrudates containing different starch types. Carbohydrate Polymers, 79(3), 592–600. Scholar Publishing
Lazou, A., & Krokida, M. (2010). Structural and textural characterization of corn and rice extrudates containing different starch types. Carbohydrate Polymers, 79(3), 592–600.
Li, C., Zhu, F., & Wang, S. (2022). Effects of high-amylose wheat on resistant starch and glycaemic response. Food Chemistry, 373, 131457. https://doi.org/10.1016/j.foodchem.2021.131457.
Li, J., Vasanthan, T., & Hoover, R. (2021). Amylose content and its influence on textural attributes of wheat and rice products. Starch/Stärke, 73(9–10), 2100014. https://doi.org/10.1002/star.202100014.
Liu, H., Wang, L., & Chen, S. (2022). Synergistic effects of multi-source starch blends on the physicochemical and rheological properties of dough systems. Food Hydrocolloids, 125, 107393.
Liu, Q., Donnell, R., & Copeland, L. (2019). Effects of starch structure and granule size on gelatinization and pasting properties. Carbohydrate Polymers, 206, 225–234.
Liu, W., Yu, S., & Guo, X. (2021). Effects of hydration and mechanical energy on the formation of glutenin macropolymers in wheat dough. Food Hydrocolloids, 112, 106338.
Ma, Y., Jin, Y., & Xiao, Y. (2025). A comprehensive review of gluten-free foods: Addressing quality defects and exploring novel enrichment strategies. Food Hydrocolloids, 173, 112261. https://doi.org/10.1016/j.foodhyd.2025.112261
Marco, C., & Rosell, C. M. (2023). Strategies for mimicking gluten functionality in gluten-free bakery systems. Food Hydrocolloids, 139, 108578. https://doi.org/10.1016/j.foodhyd.2023.108578.
Martínez, M. M., et al. (2014). Starch-protein interactions in gluten-free flours and their effect on dough rheology and bread texture. DOI: 10.1016/j.foodhyd.2013.05.005.
Mazzola, A. M., Zammarchi, I., Valerii, M. C., Spisni, E., Saracino, I. M., Lanzarotto, F., & Ricci, C. (2024). Gluten-free diet and other celiac disease therapies: Current understanding and emerging strategies. Nutrients, 16(7), 1006. https://doi.org/10.3390/nu16071006
Monteiro, M. K. S., Piler, C. W., & Ascheri, J. L. R. (2021). Technological performance of rice-based gluten-free breads as affected by amylose content. LWT – Food Science and Technology, 146, 111452. https://doi.org/10.1016/j.lwt.2021.111452.
Morales-Polanco, M., Flores, R. A., & Rendón-Villalobos, R. (2020). Influence of starch composition on gluten-free bread quality. Food Hydrocolloids, 108, 106018.
Mordor Intelligence: https://www.mordorintelligence.com/pt/industry-reports/brazil-gluten-freefoods-beverages-market-industry. Numfor, F. A., Awoyale, W., & Ikuomola, D. S. (2022). Functional and rheological characteristics of cassava (tapioca) starch and its application in food systems. International Journal of Biological Macromolecules, 208, 654–663. https://doi.org/10.1016/j.ijbiomac.2022.03.007. Scholar Publishing
Pareyt, B., Finnie, S., & Delcour, J. A. (2021). Lipid–gluten–starch interactions in bread dough: Impact on structure and freshness. Food Chemistry, 342, 128314.
Papagianni, E., Kotsiou, K., Matsakidou, A., Biliaderis, C. G., & Lazaridou, A. (2024). Development of “clean label” gluten-free breads fortified with flaxseed slurry and sesame cake: Implications on batter rheology, bread quality and shelf life. Food Hydrocolloids, 150, 109734.
Perera, A., & Hoover, R. (2019). Influence of amylose content on retrogradation and digestibility of starch-based systems. Food Chemistry, 298, 125050.
Ribeiro, A. G., et al. (2018). Physical and sensory properties of gluten-free pasta made with amaranth flour, cassava starch and xanthan gum. Food Science and Technology (Campinas), 38(2), 307-315. DOI: 10.1590/fst.08917.
Ribotta, P. D., & León, A. E. (2020). Lipid functionality in breadmaking: Gas cell stabilization and crumb softness. Journal of Cereal Science, 94, 102998. https://doi.org/10.1016/j.jcs.2020.102998.
Ronda, F., & Caballero, P. A. (2022). Hydrocolloids and alternative proteins as gluten replacers: Current trends and future perspectives. Trends in Food Science & Technology, 120, 92–108.
Rosell, C. M., et al. (2011). Strategies for developing gluten-free products. DOI: 10.1007/s11947.
Sá, G. D. S., & Masson, M. L. (2019). Gluten-free bread: Multiple strategies and new trends for development. Food Science and Technology (Campinas), 39(3), 543-553. DOI: 10.1590/fst.31318.
Russell, L. A., Alliston, P., Armstrong, D., Verdu, E. F., Moayyedi, P., & Pinto-Sanchez, M. I. (2025). Micronutrient deficiencies associated with a gluten-free diet in patients with celiac disease and non-celiac gluten or wheat sensitivity: A systematic review and meta-analysis. Journal of Clinical Medicine, 14(14), 4848. https://doi.org/10.3390/jcm14144848
Sajilata, M. G., Singhal, R. S., & Kulkarni, P. R. (2006). Resistant starch — A review. Comprehensive Reviews in Food Science and Food Safety, 5(1), 1–17. https://doi.org/10.1111/j.1541-4337.2006.tb00076.x.
Shewry, P. R., & Halford, N. G. (2022). The structure, properties, and biosynthesis of gluten proteins. Annual Review of Food Science and Technology, 13, 1–23. https://doi.org/10.1146/annurev-food-052221-093906.
Simón E, Molero-Luis M, Fueyo-Díaz R, Costas-Batlle C, Crespo-Escobar P, Montoro-Huguet MA. (2023). The Gluten-Free Diet for Celiac Disease: Critical Insights to Better Understand Clinical Outcomes. Nutrients. V.16; n. 15 (18):4013. doi: 10.3390/nu15184013. PMID: 37764795; PMCID: PMC10537989.
Singh, N., Kaur, A., & Noda, T. (2020). Potato starch: Morphology, structure, and applications. Food Hydrocolloids, 103, 105696. https://doi.org/10.1016/j.foodhyd.2020.105696.
Tester, R. F., Karkalas, J., & Qi, X. (2004). Starch structure and digestibility: Enzyme–substrate relationship. Cereal Chemistry, 81(1), 24–37. https://doi.org/10.1094/CCHEM.2004.81.1.24.
Tester, R. F., Karkalas, J., & Qi, X. (2004). Starch structure and digestibility enzyme–substrate relationship. Cereal Chemistry, 81(1), 24–37. https://doi.org/10.1094/CCHEM.2004.81.1.24. Scholar Publishing Tahmouzi, S., Meftahizadeh, H., Eyshi, S., Mahmoudzadeh, A., Alizadeh, B., Mollakhalili-Meybodi,
N., & Hatami, M. (2023). Application of guar (Cyamopsis tetragonoloba L.) gum in food technologies: A review of properties and mechanisms of action. Food Science & Nutrition, 11, 4869–4897. https://doi.org/10.1002/fsn3.3383.
Tingting Hong, Dan Xu, Yamei Jin, Fengfeng Wu, Guidong Huang, Xianfeng Zhong, Junhui Zhang, Xueming Xu (2025) Gluten protein transformations during bread processing: Molecular and microstructural analysis, LWT, v.217, 117341, doi.org/10.1016/j.lwt.2025.117341.
Tosi, P., Masci, S., & Barro, F. (2020). Disulfide bonding and the formation of glutenin polymers. Frontiers in Plant Science, 11, 572814. https://doi.org/10.3389/fpls.2020.572814.
Tudela, R., et al. (2021). Consumer insights on gluten-free bakery products: A comparison between Italy, UK, and Spain. Journal of Food Products Marketing, 27(2), 111-126. DOI: 10.1080/10454446.2021.1880387.
Wang, L. (2024). Impact of amylose/amylopectin ratio on starch pasting and functional properties: A review. Food Hydrocolloids, 150, 109109. https://doi.org/10.1016/j.foodhyd.2024.109109.
Wang, P., Tao, H., & Zhao, X. (2020). Impact of ascorbic acid and redox balance on the structure of gluten proteins in dough. Food Chemistry, 328, 127141.
Waterschoot, J., Gomand, S.V., Fierens, E. and Delcour, J.A. (2015), Starch blends and their physicochemical properties. Starch - Stärke, 67: 1-13. https://doi.org/10.1002/star.201300214.
Wieser, H., & Koehler, P. (2021). Gluten proteins and their role in determining dough viscoelasticity. Food Chemistry, 356, 129692. https://doi.org/10.1016/j.foodchem.2021.129692.
Wójtowicz, A., Oniszczuk, A., & Mitrus, M. (2020). Extrusion-cooking of starch-based snacks enriched with proteins: Structure and textural properties. Processes, 8(3), 354. https://doi.org/10.3390/pr8030354.
Xiong K, Wang J, Kang T, Xu F, Ma A. Effects of resistant starch on glycaemic control: a systematic review and meta-analysis. Br J Nutr. 2021 Jun 14;125(11):1260-1269. doi: 10.1017/S0007114520003700. Epub 2020 Sep 22. PMID: 32959735.
Yuan, Y., Chen, L., & Liu, Q. (2023). Structural and functional characteristics of rice starch and its use in hypoallergenic and gluten-free foods. Food Hydrocolloids, 136, 108253.
Zavareze, E. D. R., & Dias, A. R. G. (2021). Functional properties of cereal starches in food applications: Corn, wheat, and others. Cereal Foods World, 66(5), 244–256. https://doi.org/10.1094/CFW-66-5-0244.
Zavareze, E. R., & Dias, A. R. G. (2011). Impact of heat-moisture treatment and annealing on starches: A review. Carbohydrate Polymers, 83(2), 317–328.
Zhang, Y., Chen, X., & Li, M. (2023). Starch retrogradation and bread staling: Mechanisms and control strategies. Food Chemistry, 404, 134673.
Zhou, Y., Pareyt, B., & Delcour, J. A. (2023). Lipid roles in cereal products: From dough rheology to Scholar Publishing bread freshness. Comprehensive Reviews in Food Science and Food Safety, 22(1), 78–98. https://doi.org/10.1111/1541-4337.13054.
Zhu, F., Cai, Y., & Sun, M. (2020). Starch digestibility and glycaemic index: The role of amylose and amylopectin. Trends in Food Science & Technology, 96, 68–84.
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