Pattern of Alpha Fetoprotein and Activities of Liver Enzymes in Type 2 Diabetic Patients Attending Nnamdi Azikiwe University Teaching Hospital Nnewi, Anambra State, Nigeria.
DOI:
https://doi.org/10.14738/jbemi.85.10781Keywords:
Type 2, Diabetes, Mellitus, ALT, AST, AFP.Abstract
Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia, which has become health problem of global interest, linked to renal disease. The study is to assess the activities of some liver enzymes and alpha fetoprotein level in type 2 diabetic patients. 81 participants comprised 38 diabetics and 43 controls were randomly recruited. Informed, oral and written consent was obtained from the participants. Ethical approval was obtained. 6mls of fasting blood samples were collected. Plasma glucose, alpha feto-protein (AFP) levels; serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma glutamyltransferase (GGT) activities were analyzed using standard laboratory methods. The results showed significantly increased plasma glucose level in the diabetics than in controls (p=0.000), as well as in male diabetics than in female diabetics (p=0.048). But, the mean age, AFP, ALT, AST, ALP and GGT activities were the same in the diabetics and the controls; and in male and female diabetics at p>0.05 in each case. Relatively strong positive significant correlation was observed between ALT Vs AST (r=0.566; p=0.000) and a weaker negative association exist between ALT Vs Age (r= -0.353; p=0.022) in diabetics but no associations were observed with other parameters studied. In conclusion the positive association between ALT and AST may reveal transaminase derangements in liver status of type 2 diabetic subjects studied.
References
Akter, S., Rahman, M.M., Abe, S.K., Sultana, P. (2014). Prevalence of diabetes and prediabetes and their risk factors among Bangladeshi adults: a nationwide survey. Bulletin of World Health Organization; 92:204-213A.
Al-Dahhan, N.A.A. (2015). The Association between Liver Enzymes and Duration of Type2 Diabetes Mellitus. International Journal of Research Studies in Biosciences; 3(11):60-66.
Alzahrani, S.H., Baig, M., Bashawi, J.I., Aashi, M., Shaibi, F.K., Alqami, D.A.(2019). Prevalence and Association of Elevated Liver Transaminases in Type 2 Diabetes Mellitus Patients in Jeddah, Saudi Arabia. Cureus; 11(7): e5166.
Analike, R.A., Ihim, A.C., Iweanya, S.C., Ogbodo, E.C., Onah, C.E., Asomugha, A.L., Amah, A.K., Obi, P.C., Nwobodo, E.I., Timothy, C.O. (2019). Assessment of glycated haemoglobin, total protein and albumin levels in patients with type 2 diabetes mellitus visiting NAUTH, Nnewi. Indian Journal of Pathology and Oncology; 6(4):700-703.
Bergmeyer, H.U., Bernt, E. (1974). Determination of glucose with glucose oxidase and peroxidase. In: HU Bergmeyer (Ed.), Methods of enzymatic analysis, VerlagChemie-Academic Press, New York: 1205-1215.
Bessey, O.A., Lowry, O.H., Brock, M.J. (1946). One point colorimetric method of determining alkaline phosphatase in serum or plasma. Journal of Biological Chemistry; 164:3217.
Bragg, F. (2017). Association between diabetes and cause-specifc mortality in rural and urban areas of China. Journal of American Medical Association; 317:280–289.
Chen, Y., Zhao, Y., Feng, L., Zhang, J., Zhang, J., Feng, G. (2016). Association between alpha-fetoprotein and metabolic syndrome in a Chinese asymptomatic population: a cross-sectional study. Lipids in Health and Disease; 15:85.
Crook, M.A. (2012). Plasma enzymes in diagnosis. In: Clinical Biochemistry and Metabolic Medicine, Hodder and Stoughton Ltd, London, 8th Edition, Pp. 275-288.
Ertle, J.M., Heider, D., Wichert, M., Keller, B., Kueper, R., Hilgard, P., Gerken, G., Schlaak, J.F. (2017). A combination of α-fetoprotein and des-γ-carboxyprothrombin is superior in detection of hepatocellular carcinoma. Digestion; 87 (2): 121–131.
Forouzanfar, M. H. (2016). Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet; 388:1659–1724.
Garcia-Caballero, M., Reyes-Ortiz, A., Martínez-Moreno, J.M., Toval-Mata, J.A. (2014). Glycemic and lipid metabolic disorders in diabetic and non-diabetic patients BMI <35 or >35 before gastric bypass. Nutricion hospitalaria; 29:1095-1102.
Getnet, T., Sintayehu, A., Alebachew, F., Molla, A. (2019). Prevalence of liver function test abnormality and associated factors in type 2 diabetes mellitus: a comparative cross-sectional study. The Journal of the International Federation of Clinical Chemist and Laboratory Medicine; 30(3):303-316.
Ghimire, S., Shakya, S., Shakya, J., Acharya, P., Pardhe, B.D. (2018). Abnormal Liver parameters among Individuals with Type 2 Diabetes Mellitus Nepalese Population. Biochemistry and Pharmacology (Los Angel); 7: 243.
Gouri, A., Dekaken, A., Rouabhia, S., Bentorki, A.A., Yakhlef, A. (2013). Transaminases profile in Algerian patients with type 2 diabetes mellitus [Article in French].Immuno-analyse and Biologie Specialisee; 28:25-29.
Hall, V., Thomsen, R.W., Henrikseen, O. (2011). Diabetes in Sub-Saharan Africa 1999-2011. Epidemiology and Public Health Implications: a systemic review. BMC Public Health; 11:564.
IDF. (2019). Diabetes facts & figures:The IDF Diabetes Atlas Ninth edition 2019. Last update: 12/02/2020. Accessed March 2021. Available: https://www.idf.org/aboutdiabetes/what-is-diabetes/facts-figures.html 9
Idris, A.S., Mekky, K.F.H., Abdalla, B.E.E., Khalid Altom Ali, K.A. (2011). Liver function tests in type 2 Sudanese diabetic patients. International Journal of Nutrition and Metabolism; 3(2):17-21.
International Diabetes Federation: IDF Diabetes Atlas; 8th edition. Belgium: International Diabetes Federation, 2017.
Islam, S., Rahman, S., Haque, T., Sumon, A.H., Ahmed, A.Z.M., Ali, N. (2020). Prevalence of elevated liver enzymes and its association with type 2 diabetes: A cross-sectional study in Bangladeshi adults. Endocrinology, Diabetes and Metabolism; 3:e00116.
Lala, V., Goyal, A., Bansal, P., Minter, D.A. (2020). Liver function tests, Stat Pearls publishing LLC. Available from: http://www.ncbi.nlm.nih.gov/books/NBK482489
Lin, X., Xu, Y., Pan, X., Xu, J., Ding, Y., Sun, X., Song, X., Ren, Y., Shan, P. (2020). Global, regional, and national burden and trend of diabetes in 195 countries and territories: an analysis from 1990 t0 2025. Scientific Reports; 10:14790.
Mahesh, B.M., Chandrashekhar, M.I., Mamatha, T.M., Satish, K.W., Deepak, S.H. (2016). Evaluation of Biochemical Markers Serum Amylase and Serum Lipase for the Assessment of Pancreatic Exocrine Function in Diabetes Mellitus. Journal of Clinical and Diagnostic Research; 10(11): BC01-BC04.
Makol, A., Kanthaje, S., Dhiman, R.K., Kalra, N., Chawla, Y.K., Chakraborti, A. (2018). Association of liver cirrhosis severity with type 2 diabetes mellitus in hepatocellular carcinoma. Experimental Biology and Medicine; 243: 323–326.
Mantovani, A., Targher, G. (2017). Type 2 diabetes mellitus and risk of hepatocellular carcinoma: spotlight on nonalcoholic fatty liver disease. Annals of Translational Medicine; 5(12):270.
Marzinke, M.A., Dufour, D.R.(2020). Laboratory diagnosis of liver disease : in Contemporary Practice in Clinical th Chemistry; 4 Edition, Pp 545-559.
Moscatiello, S., Manini, R., Marchesini, G. (2007). Diabetes and liver disease: an ominous association. Nutrition, Metabolism and Cardiovascular Disease; 17:63-70.
Naing L, Winn T, Rusli B (2006). Practical issues in calculating sample size for prevalence studies. Archives of orofactual science. (1): 9-14.National AIDS Control Agency (NACA) (2011). Report on HIV prevalent rate of 3.1% in Nigeria.
Ogbodo, E.C., Okafor, C.C., Ogah, H.G.O., Ezeugwunne, I.P., Igwebuobi, C.F., Okezie, A.O., Agada, U.N., Amah, A.K., Odumodu, I.O. (2019). Thyroid Hormone Profiling and Enzymatic Antioxidant Status in Diagnosis and Management of Type-Ii-Diabetes Mellitus: A Review of Literature. World Journal of Pharmaceutical and Life Sciences; 5(12):6-21.
Papatheodorou, K., Papanas, N., Banach, M., Papazoglou, D., Edmonds, M. (2016). Complications of Diabetes 2016. Journal of Diabetes Research; 2016: 6989453.
Peer, N., Kengne, A-P., Motala, A.A., Mbanya, J.C. (2014). Diabetes in the Africa region: an update. Diabetes Research and Clinical Practice; 103:197–205.
Policardo, L. (2015). Efect of diabetes on hospitalization for ischemic stroke and related in-hospital mortality: a study in Tuscany, Italy, over years 2004–2011. Diabetes Metabolism and Research Reviews; 31:280–286.
Reitman, S., Frankel, S.A. (1957). Colorimetric method for aspartate and alanine aminotransferases in serum. American Journal of Clinical Pathology; 28:56-63.
Sharma, U., Pal, D., Prasad, R. (2014). Alkaline Phosphatase: An Overview. Indian Journal of Clinical Biochemistry; 29(3):269-278.
Szasz, G. (1969). A Kinetic Photometric Method for Serum Gamma-glutamyl Transpeptidase. Clinical Chemistry; 75: 124-136.
Traupe, T., Gloekler, S., de Marchi, S.F., Werner, G.S., Seiler, C. (2010). Assessment of the human coronary collateral circulation .Circulation; 122: 1210-1220.
Nwanguma, E. D. (2021). Pattern of Alpha Fetoprotein and Activities of Liver Enzymes in Type 2 Diabetic Patients Attending Nnamdi Azikiwe
Wannamethee, S.G., Lennon, L., Shaper, A.G.(2005). Hepatic enzymes, the metabolic syndrome, and the risk of type 2 diabetes in older men. Diabetes Care; 28:2913-2918.
WHO (2016). Global report on diabetes. World Health Organization. Available: http://www.who.int/iris/handle/10665/204871.
Yang, J. J. (2019). Association of diabetes with all-cause and cause-specifc mortality in Asia. Journal of American Medical Association Network Open; 2:e192696.
Zheng, Y., Ley, S.H., Hu, F.B. (2018). Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nature Reviews in Endocrinology; 14 (2):88-98.
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Copyright (c) 2021 Emmanuel Chukwuemeka Ogbodo, Ifeoma Priscilla Ezeugwunne, Stephen Shehu Idris, Wurola Serah Nnaemeka, Obinna DavidIbimere, Nwanneka Victoria Elosuba, Blessing K. Myke-Mbata, Ukamaka Edward, Johnjude Chinonso Nnamdi, Eberechi D. Nwanguma
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