Pesticide-induced Biochemical and Toxicological Changes in Farmers in a South-East Metropolis of Nigeria
DOI:
https://doi.org/10.14738/bjhr.1303.11870Keywords:
Pesticide toxicity, Biochemical changes, Farmers, ConsumersAbstract
Pesticides are widely used for crop protection to increase crop yields. However, they can have significant adverse effects on farmers and possibly consumers. These effects result from the use of inappropriate spraying equipment and the failure to use personal protective equipment, especially in developing countries. This study aimed to evaluate biochemical changes in farmers who use pesticides in the Enugu metropolis of Nigeria and reasoned that if crops can assimilate pesticides from the soil, consumers may also experience similar adverse effects. The cross-sectional study involved 150 subsistence farmers in Enugu Metropolis of Enugu State, Nigeria, and 120 age-matched office workers who were not involved in farming. The biochemical parameters evaluated included lipid profiles (total cholesterol, high-density cholesterol, low-density cholesterol, very low-density cholesterol and triglycerides), electrolytes, urea, creatinine and liver enzymes (alkaline phosphatase, aspartate transaminase and alanine transaminase). All parameters were measured spectrophotometrically, except electrolytes determined with ion-selective electrodes. SPSS was used to analyze the data, with a significant value of p≤0.05. The results showed that total cholesterol, low-density lipoprotein, very low-density lipoprotein and triglycerides increased significantly in pesticide-exposed persons compared to non-exposed persons (controls). Also significantly increased in farmers were urea concentration and alanine transaminase activities. There were no significant differences in concentrations of high-density lipoprotein, creatinine, electrolytes, and aspartate transaminase activity. These results implied that pesticides can cause dyslipidemia, subclinical renal disturbances and hepatotoxicity, calling for appropriate health education for farmers and insistence on the use of personal protective equipment.
References
Sosan MB, Akingbohungbe AE, Durosinmi MA, Ojo IA. Erythrocyte cholinesterase enzyme activity and hemoglobin values in cacao farmers of southwestern Nigeria as related to insecticide exposure. Arch Environ Occup Health. 2020; 65(1): 27–33. https://www.tandfonline.com/doi/full/10.1080/19338240903390289
Manfo FPT, Moundipa PF, Déchaud H. Effect of agropesticides use on male reproductive function: a study on farmers in Djutitsa (Cameroon). Environ Toxicol. 2012; 27: 423–432. https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.20656
Shu EN, Otuu FC, Maduka I, Tilako BH, Nwadinigwe AO, Ogbodo O, Nwando O. Public health implications of continuous ethnomedical use of some plant species encountered in Enyigba Lead-Zinc mining community of Ebonyi State, Nigeria. IOSR J Environ Sci Toxicol Food Tech. 2019; 13(6): 62-68. https://www.researchgate.net/publication/361536749
Hajjar MJ. Obsolete pesticides in Saudi Arabia: problems, prevention and disposal. MedCrave Online J Toxicol. 2015; 1(2): 63–68. https://medcraveonline.com/MOJT/
Jørs E, Neupane D, London L. Pesticide poisonings in low- and middle-income countries. Environ Health Insights. 2018; 2(12): 1–3. https://pmc.ncbi.nlm.nih.gov/articles/PMC5757432/
Araoud M, Neffeti F, Douki W. Adverse effects of pesticides on biochemical and haematological parameters in Tunisian agricultural workers. J Expo Sci Environ Epidemiol. 2012; 22(3): 243–247. https://pubmed.ncbi.nlm.nih.gov/22377683/
Shahzad M, Yaqub A, Shaukat M. Effects of exposure to pesticides on blood serum components and Butyrylcholinesterase (BChE) in pesticide vendors of Punjab Province, Pakistan. Ann King Edward Med University. 2016; 2(1): 6-10. https://annalskemu.org/journal/index.php/annals/article/view/1460
Jamal F, Haque QS, Singh S. The influence of pesticides on hepatic and renal functions in occupational sprayers of rural Malihabad, Lucknow (India). Toxicol Open Access. 2016; 1(1): 1–7. https://doi:10.4172/2476-2067.1000106
Ei-Ghonaimy NM. Role of ginger (Zingiber officinale) against metalaxyl induced hepatotoxicity in male albino rats: a histological and immunohistochemical study. J Histol Histopathol. 2015; 2(9): 1-5. https://doi:10.7243/2055-091X-2-9
Hassan MF, Hussein SA, El Senosi Y, Mansour MK, Amin A. The role of lycopene as antioxidant and anti-inflammatory in protection of oxidative stress induced by metalaxyl. J Med Chem Toxicol. 2018; 3(1): 26–36. https://doi:10.15436/2575-808X-18.1890
El-Gendy K, Osman K, El-Din EE, El-Seedy A. Role of biomarkers in the evaluation of cadmium and ethoprophos combination in male mice. Environ Toxicol Pharmacol. 2019; 7(2): 103-267. https://www.sciencedirect.com/science/article/abs/pii/S1382668919301413
Mueangkhiao P, Siviroj P, Sapbamrer R, Khacha-Ananda S, Lungkaphin A, Seesen M, Jaikwang P, Wunnapuk K. Biological variation in kidney injury and kidney function biomarkers among farmers in Lamphun province, Thailand. Environ Sci Polut Res. 2020; 27(11): 12386-12394. https://link.springer.com/article/10.1007/s11356-020-07661-3
Mesgnage R, Anthoniou MN. Facts and fallacies in the debate on glyphosate toxicity. Front Public Health. 2017; 5: 316. https://pmc.ncbi.nlm.nih.gov/articles/PMC5705608/
Lingjun Y, Zilin L, Xuejing Z, Bingqin X, Yu H, Qiansi C, Baochang H, Fa C, Jing W. Exposure to organochlorine pesticides and mortality risk among individuals with diabetes: A prospective cohort analysis and network pharmacology study. Ecotoxicol Environ Safety. 2025; 305: 119274. https://www.sciencedirect.com/science/article/pii/S0147651325016197 Scholar Publishing
Kalyabina VP, Esimbekova EN, Kopylova KV, Kratasyuk VA. Pesticides: formulants, distribution pathways and effects on human health – a review. Toxicol Reports. 2021; 8: 1179-1192. https://www.sciencedirect.com/science/article/pii/S2214750021001104
El-Nahhal Y. Biochemical changes associated with long term exposure to pesticide among farmers in the Gaza Strip. Occup Dis Environ Med. 2016; 4(3): 72–82. https://www.scirp.org/journal/paperinformation?paperid=70185
Calaf GM, Bleak TC, Roy D. Signs of carcinogenicity induced by parathion, malathion, and estrogen in human breast epithelial cells (Review). Oncol Rep. 2021; 45(4): 24. https://pubmed.ncbi.nlm.nih.gov/33649804/
Aslan V, Terzi S, Yeşilcimen K. Paraoxonase 1 enzyme activity in patients with isolated coronary artery ectasia. Cureus. 2025; 17(5): e83703. https://pubmed.ncbi.nlm.nih.gov/40342642/
Adeyinka A, Patel A, Kondamudi NP. Cholinergic crisis. StatPearls [Internet] 2025. https://www.ncbi.nlm.nih.gov/books/NBK482433/ (accessed on 17/4/2026)
MINADER. (Cameroon’s Ministry of Agriculture and Rural Development, 2013) Liste Des Pesticides Homologues au CAMEROUN. Liste réservée au Grand Public, MINADER. Wikipedia (accessed on June 6, 2024).
Dowling KC, Seiber JN. Importance of respiratory exposure to pesticides among agricultural populations. Intern J Toxicol. 2002; 21(5): 371–381. https://doi:10.1080/10915810290096612
Okoroafor ON, Animoke PC, Mbegbu EC, Aronu CJ, Nwanta JA, Anene B, Okoye JO. Prevalence of Newcastle isease virus in feces of free-range turkeys in Enugu, Nigeria. Veterinary World. 2020; 13(7): 1288-1293. https://doi:10.14202/vetworld.2020.1288-1293
United Nations (UN). Enugu, Nigeria, metro area population 1950–2025. Macrotrends; 2025. Available from: https://www.macrotrends.net/ cities/21986/enugu/population
SPSS Services (2025). Cochran’s Sample Size Calculator: A Comprehensive Guide. https://spssservices.com/cochran-sample-size-calculator-guide/ (accessed 8/8/2025)
Ogbodo SO, Eke CB, Nwobodo EI, Chukwurah EF, Agama VO. Oxidative modification of lipids and lipoproteins in steady state sickle cell anemic patients from south-eastern Nigeria. Am J Pediatr. 2018; 4(4): 84-88. https://www.sciencepublishinggroup.com/article/10.11648/j.ajp.20180404.12
Ogbodo SO, Aniagolu MO, Nwobodo EI. Toxic and oxidative effects of paint chemicals on paint workers in a South-eastern State of Nigeria. Adv Toxicol Toxic Effects. 2025; 9(1): 009-013. https://www.medsciencegroup.us/articles/ATTE-9-121.php
Weatherburn MW. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 1967; 39: 971-974. https://pubs.acs.org/doi/10.1021/ac60252a045
Spierto FW, McNeil ML, Burtis CA. The effect of temperature and wavelength on the measurement of creatinine with the Jaffe procedure. Clin Biochem. 1979; 12: 18-21. https://pubmed.ncbi.nlm.nih.gov/36238/
Pothu UK, Thammisetty AK, Nekakuditi LK Evaluation of cholinesterase and lipid profile levels in chronic pesticide exposed persons. J Family Med Prim Care. 2019; 8(6):2073–2078. https://pmc.ncbi.nlm.nih.gov/articles/PMC6618179/
Ebbert JO, Jensen MD. Fat Depots, Free Fatty Acids, and Dyslipidemia. Nutrients. 2013; 5(2): 498-508. https://doi:10.3390/nu5020498
Brugel M, Carlier C, Reyes-Castellanos G, Callon S, Carrier A, Bouche O. Pesticides and pancreatic adenocarcinoma: A transversal epidemiological, environmental and mechanistic Scholar Publishing narrative review. Digest Liver Dis. 2022; 54(12): 1605-1613. https://doi:10.1016/j.dld.2022.08.023
Xia T, Li Y, Huang F, Chai H, Huang B, Li Q, et al. The triglyceride paradox in the mortality of coronary artery disease. Lipids Health Dis. 2019; 18, 21. https://pmc.ncbi.nlm.nih.gov/articles/PMC6343235/
SoppertJ, LehrkeM, MarxN, JankowskiJ, NoelsH. Lipoproteins and lipids in cardiovascular disease: from mechanistic insights to therapeutic targeting. Adv Drug Delivery Rev. 2020; 159: 24-33. https://pubmed.ncbi.nlm.nih.gov/32730849/
Ogbodo SO, Aniagolu MO, Nwobodo EI. Lipid profiles of apparently healthy inhabitants of a Metropolitan City in South-East Nigeria: setting local reference values. Intern J Biol Med Res. 2026; 17(1): 8338-8341. https://doi.org/10.65048/ijbmr.2026.17.1.8338.1007
Jones RT, Andrews PJ, Brown KE. Impact of pesticide exposure on renal biomarkers: Evidence from rural agricultural communities. Environ Toxicol Pharmacol. 2018; 59: 89–96.
Nacano BRM, Convento MB, de Oliveira AS, Castino R, Castino B, Razvickas CV, Bondan E, Borges FT. Effects of glyphosate herbicide ingestion on kidney function in rats on a balanced diet. J Bras Nefrol. 2024; 46(2): e20230043. https://pubmed.ncbi.nlm.nih.gov/38039493/
Lei Q, Jinfeng Z, Yanbo Q, Hong C, Yu C, Xiaolei Y, Hongjie L, Gang L, Jicheng L. Glyphosate based-herbicide induced nephrotoxicity through oxidative stress and the imbalance of osmotic pressure in Mice. Biomed Environ Sci. 2024; 37(12): 1453-1457. https://doi.org/10.3967/bes2024.178
Smith AH, Wright RP, Henderson TM. Evaluating renal function biomarkers in populations exposed to pesticides: A comparative study. Toxicol Letters. 2021; 345: 21–30.
Kumar J, Lind L, Salihovic S, van Bavel B, Ingelsson E, Lind PM. Persistent organic pollutants and liver dysfunction biomarkers in a population-based human sample of men and women. Environ Res. 2014; 134:251–256. https://pubmed.ncbi.nlm.nih.gov/25173059/
Ogbodo SO, Tilako BH, Shuneba IL, Shu EN. Hepatotoxicity in both occupationally and environmentally-exposed inhabitants of a lead-zinc mining community. Asian Pac J Med Toxicol. 2022; 11(3):93–103. https://scialert.net/fulltext/?doi=jbs.2020.80.87
Seddik L, Bah TM, Aoues A, Brnderdour M, Silmani M. Dried leaf extract protects against lead induced neurotoxicity in Wistar rats. Eur J Sci Res. 2010; 42(1): 139-151. https://www.researchgate.net/publication/279675840
Katuwal DR (2026). Classification of insecticides based on mode of action https://www.slideshare.net/slideshow/classification-of-insecticide-based-on-mode-ofactionpdf/256935122 (accessed on 16/4/2026)
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