Reviewing the Control of Meat pH and Light Scattering in Model Systems

Authors

  • Howard J. Swatland Department of Animal Biosciences, University of Guelph

DOI:

https://doi.org/10.14738/aivp.1305.19378

Abstract

This review explains how a computer controlled system was developed to perfuse individual meat muscle fibres with phosphate buffer to change their pH. Fibres were mounted on a frame in an optical cell on a polarizing microscope to measure their birefringence. As pH was decreased, birefringence was increased. X-ray diffraction showed that this was caused by thick and thin myofilaments in the fibres moving closer together laterally. Small disks of intact muscle were measured in a macroscopic system to show that a decrease in pH caused an increase in reflectance. When pH was increased, reflectance decreased. These model systems explain how living muscle usually gets brighter as it becomes meat.

References

Swatland, H.J. (1994). Program control of changes in the pH of biological specimens for light microscopy. Journal of Computer-Assisted Microscopy 6: 41-46.

Swatland, H.J. (1989). Birefringence of beef and pork muscle fibers measured by scanning and ellipsometry with a computer-assisted polarizing microscope. Journal of Computer-Assisted Microscopy 1: 249-262. Swatland, H. J. (2025). Reviewing the Control of Meat pH and Light Scattering in Model Systems. European Journal of Applied Sciences, Vol - 13(05).

Swatland, H.J. (1990). Effect of pH on the birefringence of skeletal muscle fibers measured with a polarizing microscope. Transactions of the American Microscopical Society 109: 361-367.

Irving, T.C., Swatland, H.J. and Millman, B.M. (1990). Effect of pH on myofilament spacing in pork measured by x-ray diffraction. Canadian Institute of Food Science and Technology Journal 23: 79-81.

Swatland, H.J. (1995). Reversible effect on pork paleness in a model system. Journal of Food Science 60: 988-991.

De Sénarmont, H. (1840). Sur les modifications que la réflexion spéculaire à la surface des corps métalliques imprime à un rayon de lumière polarisée. Annales de Chimie et de Physique 73: 337-362.

Goranson, R.W. and Adams, L.H. (1933). A method for the precise measurement of optical path-difference, especially in stressed glass. Journal of the Franklin Institute 216: 475-504.

Hartshorne, N.H. and Stuart, A. (1970). Crystals and the Polarising Microscope. 4th edit., Edward Arnold, London. pp. 308-318.

Downloads

Published

2025-09-16

How to Cite

Swatland, H. J. (2025). Reviewing the Control of Meat pH and Light Scattering in Model Systems. European Journal of Applied Sciences, 13(05), 117–127. https://doi.org/10.14738/aivp.1305.19378