Antibacterial Activity of Verticillin D Produced by Endophyte Clonostachys Rosea EC 28
DOI:
https://doi.org/10.14738/aivp.105.12997Abstract
The objective of this work was to determine the antibacterial effect of endophytic fungi of Ceiba pentandra and to identify the compound(s) responsible for this activity. The antibacterial screening of the mycelium of endophytic fungi isolated from the bark of C. pentadra was evaluated against multiresistant strains E. coli and S. aureus. This screening led to the selection of isolate EC 28 for its ability to effectively inhibit the growth of the bacterial strains tested. Analysis of morphological characters and ITS rDNA sequences identified EC 28 as Clonostachys rosea. EC 28 was cultivated and extract. From the crude extract, two compounds, ergosterol and verticillin D, were isolated and identified. The in vitro activity of each compound against the growth of five strains bacterial multiresistant were assessed. Ergosterol did not have any activity. Verticillin D was active on all strains of bacterial multiresistant E. coli 942, E. coli 4814, S. aureus 931, S. aureus 934, and K. pneumonia 815 with respective MICs of 18.75; 18.75; 18.75; 3 and 37.5 µg/ ml.
References
Nicault, M., et al., “ Exploiting the biosynthetic potential for the specialized metabolism of a soil community of Streptomyces”, Antibiotics, 2020. 9: p. 271
WHO “The future of antibiotics depends on us all”, AJPTech, 2019. 2: p. 83-86.
Van Boeckel, PT., et al., “ Global trends in antimicrobial resistance in nimals in low- and middle-income countries,” Science, 2019. 365: 6459 p. 1-7.
Toty, A., et al., 'First detection of TEM-116 and SHV-75 Production of enterobacteriaceae isolated from two Ivorian university hospitals: Case of Abidjan and Bouaké', Int Curr Microbiol App Sci, 2016, 5: p. 1-9.
V. Urumova, 'Extended-spectrum beta-lactamase producing isolates of animal Enterobacteriaceae as a potential public health risk - review', Journal Med.Vet, 2015. p. 192-207.
MacVane, HS., et al., “Emergence of ceftolozane -resistant Pseudomonas aeruginosa during therapy is mediated by a single Camp structural mutation ”,Antimicrobial Agents and Chemotherapy, 2017. 61: p. 1-4.
Haidar, G., et al., “Ceftolozane-tazobactam for the treatment of multidrug-resistant Pseudomonas aeruginosa infections clinical efficacy and evolution of resistance ”, Clin Infect Dis, 2017. 5: p.110–120.
Zhang, HW., et al., Biology and chemistry of endophytes. Nat. Pro. Rep. 2006, 23: p. 753-771.
Liu, X., et al., Antimicrobial activity of an endophytic Xylaria sp. YX-28 and identification of its antimicrobial compound 7-amino-4-methylcoumarin. Appl Microbiol Biotechnol, 2008. 78 (2): p. 241–247
Elumalai, A., et al.,’A Review on Ceiba pentandra and Its Medicinal Features’, AJPTech. 2012, 2: p. 83-86.
Asare, P. and LA. Oseni, 'Comparative evaluation of Ceiba pentandra ethanolic leaf extract, stem bark extract and the combination thereof for in vitro bacterial growth inhibition', Natural Sciences Research, 2012. 5: p. 44-50.
Tran, L., et al., 'In vitro antiplasmodial activity of antimalarial medicinal plant used in Vietnamese traditional medicine”, Journal of Ethnopharmacology, 2003. 86: p. 249-252.
Doughari, JH. And AS. Ioryue, “Antimicrobial activity of stem bark extracts of Ceiba pentandra”, Pharmacologyonline, 2009. 1: p. 1333-1340.
Elion, I. et al., “ Anti-inflammatory and analgesic effects of the aqueous extract of stem of Ceiba pentandra Gaertn', Pharmacology and Pharmacy, 2014. 5: p. 1113-1118
Diomeni, DG. et al., “Effects of methylene chloride methanol extract of Ceiba pentandra root bark in normal and induced diabetic rats streptozotocin", Indian Journal of Pharmacology, 2006. 38: p. 194-197. Alagawadi, KR. and AS. Shah, “Anti-inflammatory activity of Ceiba pentandra seed extracts”, Journal of Cell and Tissue Research, 2011. 2: p. 2781-2784.
Toghueo, RK. et al.,“Enzymatic activity of endophytic fungi of the medicinal plants Terminalia catappa,Terminalia mantaly and Cananga odorata ”, Afr. J.Bot, 2017. 109: p. 146–153.
Botton, B. et al., “ Helpful and harmful molds. Industrial Importance', Masson, 1990. p. 20-191. Mycol. Res. 2009, 113: p. 33-43.
Kidd, S. et al., 'Descriptions of Medical Mushrooms, Third Edition, 2016. 278p.
White, TB. et al.,“Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics”, Research Gate, 1990. P. 315-322.
Mullis, KB and FA. Faloona,“ In vitro specific DNA synthesis via a polymerase-catalyzed chain reaction”, Methods in Enzymology, 1987. 155: p. 335-50.
Ponce, AG. et al., 'Antibacterial activity of essential oils from native microflora of organic Swiss chard', Society of Food Science and Technology, 2003. 36: p. 679-684.
Fawole, OA. et al.,'Antimicrobial activity and mutagenic effects of twelve traditional medicinal plants used to treat food related to the gastrointestinal tract in South Africa”, South African Journal of Botany, 2009. 75 : p. 356-362 .
Santos, IP. et al.,' antibacterial activity of leaf endophytic fungi of Indigofera suffruticosa Miller (Fabaceae)', Frontiers in Mocrobilogy, 2015. 6: p. 350-365
HJ Schroers, A monograph of Bionectria (Ascomycota, Hypocreales, Bionectriaceae) and its Clonostachys anamorphs. Studies in Mycology, 2001. 46: p. 1-214.
Joshi, K. et al., “New Verticillin and Glisoprenin Analogues from Gliocladium catenulatum, a Mycoparasite of Aspergillus flavus Sclerotia, Journal of Natural Products, 1999. 62: p. 730-733.
Gao, H. et al., “Structural diversity and biological activities of the secondary novel endophyte metabolites. Molecules, 2018. 23: p. 646
Schroers, HJ. Et al., Classification of mycoparasite Gliocladium roseum to Clonostachys as C. rosea, its relationship to Bionectria ochroleuca, and notes on other gliocladium like fungi. Mycology, 1999. 91: p. 365-385
Roberti, R.A et al. Protein induction and resistance by the biological control agent Clonostachys rosea in wheat plants infected with Fusarium culmorum. Plant Sci, 2008. 175: p. 339-347.
Morandi, M.B. et al.,’Influence of application time on the establishment, survival, and ability of Clonostachys rosea to suppress Botrytis cinerea sporulation on pink debris’,Crop Prot, 2008. 27: p. 77-83. Xue, AG. et al.,‘Concentration and cultivar effects on efficacy of CLO-1 biofungicide in controlling Fusarium head blight of wheat’, Biol. Control, 2014. 73: p. 2-7.
Parck, S.H. and H.K. Kim, antibacterial activity of emulsions containing ergosterol esters of unsaturated fatty acids synthesized by lipase-mdiated transesterification. Enzyme and Microbial Technology, 2020. 139 : p. 10956-10981
Zheng, C.J. et al.,’Verticillin G, a newantibacterial compound from Bionectra byssicola’, J. Antibiot, 2007. 38: p. 61–64.
De Clercq, E., et al., Antireverse transcriptase activity of gliotoxin analogs. Biochem Pharmacol. 1978. 27: p. 635–639.
Rightsel, W. et al., Antiviral activity of gliotoxin and gliotoxin acetate. Nature, 1964. 204: p. 1333–1334.
Rodriguez, P. L., and L. Carrasco, Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol. J Virol, 1992. 66: p. 1971–1976.
Trown, P. W. and J. A. Bilello, Mechanism of action of gliotoxin: elimination of activity by sulfhydryl compounds. Antimicrobials Agents Chemother, 1972. 2 : p. 261–266.
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