Assessment of Emulsification Potentials and Bio- Diversities of Bacterial Isolates from Bonny Loading Jetty Crude Oil Polluted Soil, Bonny Island, Rivers State.
DOI:
https://doi.org/10.14738/aivp.103.11378Keywords:
Surfactants, bioremediation, emulsification, microbesAbstract
Surfactant producing microorganisms are indispensable in utility; In the present study, contaminated homogenized soil samples from Bonny Island River showed the following physicochemical properties: pH (5.67), Chloride ion (Cl-) (321±0.2 mg/g),Potassium ion (K+) (5.26±0.01mg/g), Magnesium ion(Mg2+) (2.19±0.05 mg/g), Iron II ion(Fe2+) (38.73±0.13ppm), Calcium ion (Ca2+) (4.12±0.1mg/g), Copper II ion (Cu2+) (3.2±0.01ppm), Lead II ion (Pb2+) (0.21±0.02 ppm), Phosphate ion (PO3) (6.24±0.1 mg/g), Total petroleum hydrocarbons(TPH 364±0.05 Mg/g), Total organic carbon (TOC 56.5±0.05 mg/g) and Total organic matter contents (TOM) (63.45±0.2) .Prior to the emulsification screening carried out; microbial isolation and total heterotrophic counting (CFU/g) carried out showed a total of twelve microbial distinct isolates from the polluted soil with a microbial population of 2.5 x 108 and 2.1 x 108 CFU/g of the soil. Dilution folds of 10-1 and 10-2 from the Bushnell-Hass media showed microbial population of 0.33 x 104 and 0.78 x 104CFU/g respectively. Klebsiella sp. showed emulsification indices of43.3%, 51.2%, 50 and 48.9%with diesel and coconut oil, kerosene and crude oil respectively. Pseudomonas sp. showed emulsification indices of 40, 43, 54 and 45.6% with diesel, coconut oil, kerosene and crude oil respectively. Bacillus sp. showed emulsification indices of 46, 45, 32 and 37% with diesel, coconut oil, kerosene and crude oil respectively. The present study have shown the great potential of utilization of the bacterial consortium in oil recovering from various damp oil-wells and in cost efficient bioremediation process.
References
Abu, G. and Ogiji, P. (1996).Initial test of a bioremediation scheme for the clean up of an oil–polluted water body in a rural community in Nigeria. Bioresource Technology, 58: 7 – 12.
Abu, G. and Ogiji, P. (1996).Initial test of a bioremediation scheme for the clean up of an oil–polluted water body in a rural community in Nigeria. Bioresource Technology, 58: 7 – 12
Adieze, I. (2012). Preliminary assessment of hydrocarbon-polluted soil bacterial isolates for biosurfactant production. Journal of Nigerian Environmental Society,7: 77-87.
Adieze, I., Nwabueze, R. and Onyeze, G. (2003).Effect of poultry manure on the microbial utilization of hydrocarbons in oil–polluted soil. Nigerian Journal of Microbiology.17: 12–16.
Adieze, I., Nwabueze, R. and Onyeze, G. (2003).Effect of poultry manure on the microbial utilization of hydrocarbons in oil–polluted soil. Nigerian Journal of Microbiology,17: 12–16.
Agency for Toxic Substance Development and Disease Registry (ATSDR) (2010).Documentary on Toxicological Profile of Total Petroleum Hydrocarbon Contaminations. Agency for Toxic Substances And Disease Registry, Division of Toxicoloy and Toxicology Information Branch, Atlanta Georgia.
Barnett, H. and Hunter, B. (1972). Illustrated genera of imperfect fungi. Macmillan Publishing Company, New th York. 4 edition, pg. 114-119
Brooijmans, R., Pastink, M. and Siezen, R. (2009). Hydrocarbon-degrading bacteria: the oil-spill clean-up crew. Microbial Biotechnology, 2: 587–594.
Brooijmans, R., Pastink, M. and Siezen, R. (2009). Hydrocarbon-degrading bacteria: the oil-spill clean-up crew. Microbial Biotechnology.2: 587–594.
Chikere C., Okpokwasili, G. and Chikere, B. (2006). Bacterial Diversity in Typical Crude oil Polluted Soil Undergoing Bioremediation. African Journal of Biotechnology, 8:2535-2540.
Cooper, D. and Goldenberg, B. (1987). Surface-active agents from twoBacillus species. Applied Microbiology and Biotechnology, 53(2): 224-229.
Jain, D., Collins-Thompson, D. and Lee, H. (1991).A collapsing test for screening surfactant- producing microorganisms. Journal of Microbiology Methods, 13(4): 271-279.
Leahy, J. and Colwell, R. (1990). Microbial degradation of hydrocarbons in the environment. Microbiological Reviews, 54:305-315.
Mbachu, E., chukwurah, O. and Mbachu, I. (2016).Isolation and characterization of hydrocarbon degrading fungi from used (spent) engine oil polluted soil and their use for polycyclic aromatic hydrocarbons (PAHs) degradation. Universal Journal of Microbiology Research, 4(1): 31-37.
Morikawa, M., Hirata, Y. and Imanaka, T. (2000).A study on the structure-function relationship of lipopeptide biosurfactants. Biochem.Biophys Acta, 1488 (3): 211–218.
Odu, C. and Isinguzo, S. (1979). Oil spillage and the useof infant organisms for enhanced biodegradation. The petroleum industry and the Nigerian Environment: Proceedings of 1987 International Seminar Nigerian National Petroleum Corporation. Pp. 133-138.
Okpokwasili, G. and Amanchukwu, S. (1988). Petroleum hydrocarbon degradation by Candida sp. Environmental International, 14: 243 - 247.
Onugbolu, C. and Adieze I. (2016). Assessment of emulsification potentials of hydrocarbon utilizing fungi isolates isolated from a crude oil polluted at FUTO, Owerri. Nigerian Journal of Microbiology, 30(1): 3225-3234.
Rahman, K., Thahira-Rahman, J., Mcclean, S., Marchant, R. and Banat, I. M. (2002). Rhamnolipidbio surfactant production by strains of Pseudomonas aeruginosa using low cost raw materials. Biotechnology Progress, 18: 1277 – 1281.
Satpute, S., Bhawsar, B., Dhakephalkar, P. and Chopade, B. (2008). Assessment of different screening methods for selecting biosurfactant producing marine bacteria. Indian Journal of Microbial Sciences, 37:243–250.
Valerro D. (2010). Environmental biotechnology: A Biosystems approach. Elseveir publishers, North Carolina, USA, Pg 549-589.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Chijioke O. Ezenwelu, Amarachukwu V. Arazu, Izuchukwu Chukwudozie, Oparaji H. Emeka
This work is licensed under a Creative Commons Attribution 4.0 International License.
