Antibiogram and Multidrug Resistance Pattern of Staphylococcus aureus from a Private Diagnostic Laboratory
DOI:
https://doi.org/10.14738/aivp.102.11840Keywords:
Antibiogram, Antibiotics, Resistance, Staphylococcus aureusAbstract
Multidrug-resistant Staphylococcus aureus have emerged as a major clinical and therapeutic dilemma in hospitals in Nigeria. To describe the prevalence of infections attributable to multidrug-resistant Staphylococcus aureus among patients at a private Diagnostic Laboratory in Rivers State of Nigeria. Isolates were collected and sub-cultured in the Medical Microbiology Laboratory of the University of Port Harcourt. Bacterial isolates were identified using standard microbiological methods. Fifty (50) target organisms (Staphylococcus aureus) were isolated from different clinical sources were identified. Of these isolates 38% and 62% were recovered from male and female patients respectively. The largest proportions of patients were from age-group 21-30 years (47.5%) followed by 31-40 years (18.2%). The decreasing order of isolates sources ranged from urine 16(32%), HVS 12(24%), Ear 6(12%), Hair 6(6%) and Toe 5(10%). The isolates showed high resistance to Cefoxiitin (FOX), Ceftazidime (100%), Ciprofloxacin (100%), Cefolaxime (100%), Cloxacillin (100%), gentamicin (12%) and erythromycin (36.8%). The average multi-drug resistance was 100% ranging from all the isolates. Staphylococcus aureus infections caused by multi-drug resistant, (isolates resistant to at least one agent in three or more antibiotic classes) Staphylococcus aureus among Patient. To prevent further emergence and spread of MDR Staphylococcus aureus, rational use of antibiotics and regular monitoring of antimicrobial resistance patterns are essential and mandatory.
References
Wang, L., Hu, C., and Shao, L. The antimicrobial activity of nanoparticles: present situation and prospects for the future. Int. J. Nanomed. 2017. 12, 1227–1249.
Lee, H. Methicillin (Oxacillin)-Resistant Staphylococcus aureus Strains isolated from major food animals and their potential transmission to humans. Applied and Environmental Microbiology, 2003, 69(11):6489–6494.
Fisher, E. A., and Paterson, G. K. Prevalence and characterization of methicillin resistant staphylococci from bovine bulk tank milk in England and Wales. J. Glob. Antimicrob. Resist. 2020, 22:139 – 144.
Shah, D., Wasim, S. and Farhan E. Comparison of oxacillin and cefoxitin for the detection of mecA gene to determine methicillin resistance in coagulase negative staphylococci (CoNs). Journal of the College of Physicians and Surgeons Pakistan. 2017, 27(8):520-522.
Foster, T. J. Potential for vaccination against infections caused by Staphylococcus aureus. Vaccine,1991, 9:221–227.
Saba, C., Amenyona, J. and Kpordze, S. Prevalence and pattern of antibiotic resistance of Staphylococcus aureus isolated from door handles and other points of contact in public hospitals in Ghana. Antimicrobial Resistance and Infection Control, 2017, 6:44.
Serwecinska, L. Antimicrobials and Antibiotic-Resistant Bacteria: A Risk to the Environment and to Public Health. Water.2020, 12:3313.
Cesur, S. and Demiröz, P. Antibiotics and the mechanisms of resistance to antibiotics. Medical Journal of Islamic World Academy of Sciences, 2013, 21(4):138-142.
Schnitt, T., and Lienen, H., Wichmann-Schauer, and Tenhagen, B. The occurrence of methicillin-resistant nonaureus staphylococci in samples from cows, young stock, and the environment on German dairy farms. J. Dairy Sci. 2021, 104:4604–4614.
Akanbi, O., Njom, H., Fri, H. Anthony C. and Anna M. Antimicrobial Susceptibility of Staphylococcus aureus Isolated from RecreationalWaters and Beach Sand in Eastern Cape Province of South Africa. Int. J. Environ. Res. Public Health 2017, 14: 1001;
Cheesbrough, M. District Laboratory Practice in Tropical countries Part 1&2. Second edition update, Cambridge University Press. 2002, (62): 97-115, 132-143.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; fifteenth informational supplement, Clinical and Laboratory Standard Institute Wayne, Pa. 2014, M100- S15, 25: 1.
Agbagwa, O.E., and Jirigwa, C.E. Antibiotics resistance and Plasmid Profile of Staphylococcus aureus from wound swabs in Port Harcourt Nigeria. Current Research in Bacteriology, 2015; 8 (3):70-76.
Agbagwa, O.E and Edje, O.R.. ‘Methicillin Resistant Staphylococcus aureus in Wounds Swab of Patients attending a Public Hospital in Warri Delta State, Nigeria’. British Microbiology Research Journal. 2016, 11(3):1-8.
Hanif, E. John, A. and Hassan, S.A. Evaluation of antibiotic resistance pattern in clinical isolates of Staphylococcus aureus. Pak. J. Pharm. Sci. 2019, 32: 1749–1753.
Nwoire A., Madubuko E., Eze U. A., Oti-Wilberforce R., Azi S., Ibiam G., Egwu H., Okereke E. and Obi I. Incidence of Staphylococcus aureus in clinical specimens in Federal Teaching Hospital, Abakaliki, Ebonyi State. Merit Research Journal of Medicine and Medical Sciences 2013, (3) :043-046.
Soe, P., Han, W., Sagili, K., Satyanarayana, S., Shrestha, P. Htoon, T. and Tin, H. High Prevalence of Methicillin-Resistant Staphylococcus aureus among Healthcare Facilities and Its Related Factors in Myanmar (2018–2019). Trop. Med. Infect. Dis. 2021, 6:70.
Ghia, C., Waghela, S. and Rambhad, G. A Systemic Literature Review and Meta-Analysis Reporting the Prevalence and Impact of Methicillin-Resistant Staphylococcus aureus Infection in India. Infect. Dis. Res. Treat. 2020, 2:13.
Daniyan, S.Y., Galadima, M., Ijah, U. J.J., and Odama, L. E. Antibacterial activity of three plant extracts used in Nigeria folkloric medicine against hospital isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA). International Resaerch Journal of Pharmacy, 2011, 3: 74-81.
Akpaka, P.E., Kissoon, S., Swanston, W.H. et al. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus isolates from Trinidad & Tobago. Ann Clin Microbiol Antimicrob 2006, 5:16.
Ocan, M, Bwanga, F, Bbosa, G.S, Bagenda, D, Waako, P, Ogwal-Okeng, J. and Obua, C. Patterns and predictors of self-medication in northern Uganda. PLoS One, 2014,9:92323–30.
Abdelghafar, A., Yousef, N. and Askora, M. Prevalence and antimicrobial susceptibility patterns of Staphylococcus aureus isolated from different clinical sources. Zagazig J. Pharm. Sci. 2020, 29, 1–8.
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Copyright (c) 2022 Obakpororo Ejiro Agbagwa, Chukwuemeka Joy Chineye, Selepiri Jemina Horsefall
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