In vitro Regeneration of Gerbera (Gerbera jamesonii) in SAU Tissue Culture Medium
DOI:
https://doi.org/10.14738/aivp.1402.20129Keywords:
Gerbera, regeneration, SAU TC medium, MS (1962) mediumAbstract
In vitro regeneration potentiality of Gerbera (Gerbera jamesonii) flower was evaluated in ammonium nitrate free SAU Plant tissue culture medium. The most population MS (1962) medium and MS powder (Duchefa Biocheme, The Netherland) were used as two check treatments. Data were collected on callus induction, days to shoot initiation, multiple shoot regeneration, shoot length, number of leaves, root number and root length. Among the treatments, MS powder (T1) showed the best shoot performance for producing the highest number of shoots (23.00), longest shoots (7.40 cm) and maximum leaves (5.33) at 42 days after inoculation. The second highest performance showed by the SAU Tissue Culture medium which was very close to MS powder and non significant for most of the traits under study. The SAU TC showed the highest root number (7.33) and length (6.97 cm). Regenerated plantlets showed 95% survival under controlled conditions and 75% in field acclimatization. The findings validate the SAU TC medium as a safe, economical and effective alternative to MS media for large-scale in vitro propagation of Gerbera in Bangladesh.
References
Jaleel, C. A., Gopi, R., Sankar, B., Manivannan, P., Kishorekumar, A., Sridharan, R., & Panneerselvam, R. (2008). Responses of Withania somnifera to water stress. Plant Physiology and Biochemistry, 46(2), 527-533.
Thul, S. T., Pawar, R. R., & Kharde, R. B. (2012). An efficient protocol for in vitro plant regeneration of Gerbera jamesonii hybrida. International Journal of Pharma and Bio Sciences, 3(1), P-160-P-165.
Brishty, U. R., Ahmmed, A. N. F., Sultana, N., & Huda, N. (2022). Survey on Socio-Economic Status of Gerbera Growers in Bangladesh. Asian Journal of Agriculture and Allied Sciences, 5(1), 12-19.
Rout, G. R., Mohapatra, A., & Jain, S. M. (2006). Micropropagation of ornamental plants: a critical review. Environmental and Experimental Botany, 55(3), 163-181.
Murashige, T., Serpa, M., & Jones, J. B. (1974). Clonal propagation of Gerbera through tissue culture. HortScience, 9(3), 175–177.
George, E. F., & Sherrington, P. D. (1984). Plant propagation by tissue culture: Handbook and bibliography. Exegetics Ltd.
Pierik, R. L. M., Steegmans, H. H. M., & Hendriks, J. (1975). Optimization of Gerbera plantlet production from excised capitulum explants. Scientia Horticulturae, 3(4), 351–357.
Hasan, M., Hossain, M. M., Sarker, B. C., & Rahman, M. M. (2010). In vitro regeneration of Gerbera (Gerbera jamesonii) through organogenesis. Bangladesh Journal of Agricultural Research, 35(1), 1–10.
Begum, F. (2017). In vitro regeneration of Gerbera using different explants and hormonal combinations. IJRDO - Journal of Biological Science, 3(6), 12–18.
Hoque, M., Koly, M., Zarin, Z., Sarker, C., Sultan, T., & Ali, M. (2025). Innovation of a new plant tissue culture medium without utilizing explosive chemical ammonium nitrate (NH₄NO₃). Discoveries in Agriculture and Food Sciences, 13, 64–76.
Gantait, S., & Mahanta, M. (2021). Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of Gerbera. Journal of Genetic Engineering and Biotechnology, 19(1), 175.
Barbosa, M. H. P., Pinto, J. F. B. P., Pinto, C. A. B. P., & Innecco, R. (1994). In vitro propagation of Gerbera jamesonii Bolus ex Hook cv. Appel Bloesem using young capitulum. Revista Ceres, 41, 386–395. Scholar Publishing
Laliberté, S., Chrétien, L., & Vieth, J. (1985). In vitro plantlet production from young capitulum explants of Gerbera jamesonii. HortScience, 20(1), 137–139.
Kumar, S., & Kanwar, J. K. (2007). Plant regeneration from callus and leaf explants of Gerbera jamesonii. Biologia Plantarum, 51(4), 786–790.
Shabbir, K., Ahmad, T., Hafiz, I. A., Hussain, A., Abbasi, N. A., & Ahmad, J. (2012). In vitro regeneration of Gerbera jamesonii cv. Sunglow. African Journal of Biotechnology, 11, 9975–9984.
Celenza, J. L., Grisafi, P. L., & Fink, G. R. (1995). A pathway for lateral root initiation in Arabidopsis thaliana. Genes & Development, 9(16), 2131–2142.
Kozai, T., & Smith, M. A. L. (1995). Environmental control in plant tissue culture—General introduction and overview. In T. Kozai, M. A. L. Smith, & J. Aitken-Christie (Eds.), Automation and environmental control in plant tissue culture (pp. 1–20).
Bhojwani, S. S., & Razdan, M. K. (1992). Plant tissue culture: Theory and practice (2nd ed.). Elsevier.
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Copyright (c) 2026 Zannatun Naium Shoma, Md. Ekramul Hoque, Talha Zubair Masror, Md. Jamal Hossain, Homayra Huq
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