Tolerance Profiling of Selected BRRI Rice Varieties Against Pre-Harvest Sprouting

Authors

  • Tuhin Halder Plant Physiology Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh
  • Md. Rafiqul Islam Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh
  • Mst. Salma Pervin Plant Physiology Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh
  • Md. Mozammel Haque Soil Science Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh

DOI:

https://doi.org/10.14738/aivp.1402.20256

Keywords:

Rice Plants, pre-harvest sprouting, seed dormancy, Variety screening, PCA, Cluster analysis, BRRI Rice Varieties

Abstract

Pre-harvest sprouting (PHS) of grains while attached to the mother plant before harvest is a major threat to lowland rice production in Bangladesh, especially in the high rainfall period at the harvesting stage. The study aimed to assess varietal differences in PHS tolerance of 35 BRRI rice varieties. A modified water-soaking technique was used to enhance PHS at 25 and 30 days after flowering (DAF). Significant variations were observed among the varieties underlying the inherent properties of PHS tolerance. At 25 DAF, most of the varieties exhibited less than 5% germination, which increased at 30 DAF. BR16, BR19, BRRI dhan36, BRRI dhan45, BRRI dhan48, BRRI dhan55, BRRI dhan84, and BRRI dhan88 showed high tolerance to PHS in both the growth stages. BRRI dhan50, BRRI dhan86, and BRRI dhan89 exhibited high susceptibility (>50% PHS). There was a strong positive correlation between PHS percentage and reduction in 1000-grain weight after 30 DAF. Clustering and principal component analysis (PCA) analysis showed that PHS tolerance is strongly dependent on genotype and seed maturity stage. PHS-tolerant BRRI rice varieties could be used as parent materials for breeding sprouting-resistant variety development. It is better to adjust the planting window for BRRI dhan50, BRRI dhan86, and BRRI dhan89 varieties so that they can avoid high rainfall during the harvesting stage.

References

Halder, T., Hoque, M. E., Islam, M. M., Ali, L., & Chowdhury, A. K. (2016). Morphomolecular Characterization of Local Boro Rice (Oryza Sativa L.) Germplasm. Bangladesh Journal of Plant Breeding and Genetics, 29(2), 01–09. https://doi.org/10.3329/bjpbg.v29i2.33941

Bangladesh Bureau of Statistics. (2024). Yearbook of Agricultural Statistics 2024. Statistics and Informatics Division, Ministry of Planning, Government of the People’s Republic of Bangladesh. https://bbs.portal.gov.bd/sites/default/files/files/bbs.portal.gov.bd/page/1b1eb817_9325_4354_a756_3d18412203e2/2025-06-09-12-39-7f8d0b17c93237117fdd6567de090928.pdf

Dupont, F., & Altenbach, S. (2003). Molecular and biochemical impacts of environmental factors on wheat grain development and protein synthesis. Journal of Cereal Science, 38(2), 133–146. https://doi.org/10.1016/s0733-5210(03)00030-4

Halder, T., Biswas, A., Barman, H. N., Akter, S., Shupta, S. A., & Pervin, M. S. (2025). Thermal Requirement for the Phenological Development and yield of Rice as Influenced by Seeding Date. Bangladesh Rice Journal, 27(2), 39–50. https://doi.org/10.3329/brj.v27i2.77303

Huke RE and Huke EH 1990. Rice: Then and now. International Rice Research Institute, Philippines

Tejakhod, S., & Ellis, R. H. (2018). Effect of simulated flooding during rice seed development and maturation on subsequent seed quality. Seed Science Research, 28(1), 72–81. https://doi.org/10.1017/s0960258517000368

Bose, L. K., Jambhulkar, N. N., Sandhamitra, P., & Patra, B. C. (2019). Identification of preharvest sprouting tolerant rice genotypes for lowland ecology. International Journal of Current Microbiology and Applied Sciences, 8(01), 1669–1673. https://doi.org/10.20546/ijcmas.2019.801.175

Li, C., Ni, P., Francki, M., Hunter, A., Zhang, Y., Schibeci, D., Li, H., Tarr, A., Wang, J., Cakir, M., Yu, J., Bellgard, M., Lance, R., & Appels, R. (2004). Genes controlling seed Scholar Publishing dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. Functional & Integrative Genomics, 4(2), 84–93. https://doi.org/10.1007/s10142-004-0104-3

Yan, D., Duermeyer, L., Leoveanu, C., & Nambara, E. (2014). The functions of the endosperm during seed germination. Plant and Cell Physiology, 55(9), 1521–1533. https://doi.org/10.1093/pcp/pcu089

Mohapatra, P. K., & Kariali, E. (2016, December 23). Management of viviparous germination in rice: a strategy for development of climate resilient rice cultivation. https://epubs.icar.org.in/index.php/OIJR/article/view/71195?utm

Yang R., M. T. Harrison, Y. Yang, C. Wang, S. Shabala, M. Huang, C. Zhao, M. Zhou, C. Sun, and K. Liu, 2025. Pre-harvest sprouting in cereals: Global incidence, impacts and mitigation strategies, Field Crops Research, 333; https://doi.org/10.1016/j.fcr.2025.110111.

Zhang, C., L. Zhou, Y. Lu, Y. Yang, L. Feng, W. Hao, Q. Li, X. Fan, D. Zhao, and Q. Liu. 2020. Changes in the physicochemical properties and starch structures of rice grains upon preharvest sprouting. Carbohydrate Polymers, 234; https://doi.org/10.1016/j.carbpol.2020.115893

Mahbub, A. A., Rahman, M. S., Khanam, M., & Gomosta, A. R. (2005). Development of preharvest sprouting tolerance screening technique in rice. International Rice Research Notes, 30(1), 39–40. https://www.researchgate.net/publication/235331639

Foolad, M. R., Subbiah, P., & Zhang, L. (2007). Common QTL Affect the Rate of Tomato Seed Germination under Different Stress and Nonstress Conditions. International Journal of Plant Genomics, 2007, 1–10. https://doi.org/10.1155/2007/97386

Gao, F., & Ayele, B. T. (2014). Functional genomics of seed dormancy in wheat: advances and prospects. Frontiers in Plant Science, 5, 458. https://doi.org/10.3389/fpls.2014.00458

Liu, D., Zeng, M., Wu, Y., Du, Y., Liu, J., Luo, S., & Zeng, Y. (2022). Comparative transcriptomic analysis provides insights into the molecular basis underlying pre-harvest sprouting in rice. BMC Genomics, 23(1), 771. https://doi.org/10.1186/s12864-022-08998-4

Mao, X., Zhang, J., Liu, W., Yan, S., Liu, Q., Fu, H., Zhao, J., Huang, W., Dong, J., Zhang, S., Yang, T., Yang, W., Liu, B., & Wang, F. (2019). The MKKK62-MKK3-MAPK7/14 module negatively regulates seed dormancy in rice. Rice, 12(1), 2. https://doi.org/10.1186/s12284-018-0260-z

Sohn, S., Pandian, S., Kumar, T. S., Zoclanclounon, Y. a. B., Muthuramalingam, P., Shilpha, J., Satish, L., & Ramesh, M. (2021). Seed dormancy and Pre-Harvest Sprouting in Rice—An updated Overview. International Journal of Molecular Sciences, 22(21), 11804. https://doi.org/10.3390/ijms222111804

Zhao, B., Zhang, H., Chen, T., Ding, L., Zhang, L., Ding, X., Zhang, J., Qian, Q., & Xiang, Y. (2022). Sdr4 dominates pre‐harvest sprouting and facilitates adaptation to local climatic condition in Asian cultivated rice. Journal of Integrative Plant Biology, 64(6), 1246–1263. https://doi.org/10.1111/jipb.13266

Lee, J., Chebotarov, D., McNally, K. L., Pede, V., Setiyono, T. D., Raquid, R., Hyun, W., Jeung, J., Kohli, A., & Mo, Y. (2021). Novel sources of Pre-Harvest sprouting resistance for Japonica rice improvement. Plants, 10(8), 1709. https://doi.org/10.3390/plants10081709

Jang, S., Kim, B., Choi, I., Lee, J., Ham, T., & Kwon, S. (2023). Fine-Mapping Analysis of the Genes Associated with Pre-Harvest Sprouting Tolerance in Rice (Oryza sativa L.). Agronomy, 13(3), 818. https://doi.org/10.3390/agronomy13030818 Scholar Publishing

Bewley, J.D., Bradford, K.J., Hilhorst, H.W.M. and Nonogaki, H. (2013) Seeds Physiology of Development, Germination and Dormancy. 3rd Edition, Springer, New York. - References -Scientific Research Publishing. (n.d.). https://www.scirp.org/reference/referencespapers?referenceid=1880188

Fang, J., Chu, C., Fang, J., & Chu, C. (2008). Abscisic acid and the pre-harvest sprouting in cereals. Plant Signaling & Behavior, 3(12), 1046–1048. https://doi.org/10.4161/psb.3.12.6606

Mares, D. J., & Mrva, K. (2014). Wheat grain preharvest sprouting and late maturity alphaamylase. Planta, 240(6), 1167–1178. https://doi.org/10.1007/s00425-014-2172-5

Soheila, N. T., & Aalami, A. (2018). Evaluation of rice cultivats based on pre-harvest sprouting tolerance. ijfcs.ut.ac.ir. https://doi.org/10.22059/ijfcs.2017.217767.654205

Barnard, A., & Booyse, M. (2017). The effect of seed treatments on the yield and yield components of various levels of sprouted wheat. Seed Science and Technology, 46(1), 53–63. https://doi.org/10.15258/sst.2018.46.1.05

Tuan, P. A., Kumar, R., Rehal, P. K., Toora, P. K., & Ayele, B. T. (2018). Molecular mechanisms underlying abscisic Acid/Gibberellin balance in the control of seed dormancy and germination in cereals. Frontiers in Plant Science, 9, 668. https://doi.org/10.3389/fpls.2018.00668

Sugimoto, K., Takeuchi, Y., Ebana, K., Miyao, A., Hirochika, H., Hara, N., Ishiyama, K., Kobayashi, M., Ban, Y., Hattori, T., & Yano, M. (2010). Molecular cloning ofSdr4, a regulator involved in seed dormancy and domestication of rice. Proceedings of the National Academy of Sciences, 107(13), 5792–5797. https://doi.org/10.1073/pnas.0911965107

Gu, X., Foley, M. E., Horvath, D. P., Anderson, J. V., Feng, J., Zhang, L., Mowry, C. R., Ye, H., Suttle, J. C., Kadowaki, K., & Chen, Z. (2011). Association between seed dormancy and pericarp color is controlled by a pleiotropic gene that regulates abscisic acid and flavonoid synthesis in weedy red rice. Genetics, 189(4), 1515–1524. https://doi.org/10.1534/genetics.111.131169

Downloads

Published

2026-04-28

How to Cite

Halder, T., Islam, M. R., Pervin, M. S., & Haque, M. M. (2026). Tolerance Profiling of Selected BRRI Rice Varieties Against Pre-Harvest Sprouting. European Journal of Applied Sciences, 14(02), 548–558. https://doi.org/10.14738/aivp.1402.20256