Therapeutic Effects of Methanol and Aqueous extract of Terminalia glaucescens (Combretaceae) on Gastro-intestinal Nematodes of Djallonke Sheep
DOI:
https://doi.org/10.14738/ejas.1403.10813Keywords:
Terminalia glaucescens, gastrointestinal nematodes, sheep, Dschang, CameroonAbstract
Background: Gastrointestinal nematodes (GIN) are a major threat to sheep productivity and endangers animal welfare worldwide particularly in developing countries. They cause loss of production through mortality, weight loss, reduced milk, meat and wool production. Thus, GIN infection is an important limiting factor or constraint in livestock production. Aim: To evaluate the anthelmintic activity of organic and aqueous extracts of stem bark of Terminalia glaucescens against gastrointestinal nematodes of sheep using varieties of in vivo tests. Materials and Methods: Fourty (40) Djallonke sheep acquired natural infection with gastrointestinal nematodes of both sexes, aged 6-10 months old and weighing between 8-15kg, used in bioassay were distributed into 5 groups (n=8). Two experiments using methanol (Groups A to E) and hot water extracts (Groups A’ to E’) were simultaneously carried out. Groups A & A’ received 1.25% dimethyl sulfoxide (DMSO) and distilled water 1ml/10 kg bodyweight (bwt) respectively, Groups B & B’ received Fenbendazole at 6.25mg/kg bwt, Groups C & C’, D & D’ and E & E’ received doses of 125, 250 and 500 mg/kg bwt of each extract. Sheep were subjected to different treatment with single dose of synthetic drug and double doses of plant extracts. Results: Methanol extract for all the doses tested was active in vivo on the adults of GIN, and reduced significantly (p<0.05) the faecal egg count (FEC) and total warm count (TWC) of the nematodes. The dose rate 500mg/kg showed the highest nematocidal activity of 77, 6% FEC and 73, 5% TWC reduction 14 days post-treatment. For hot water extract, these numbers were 65,3% and 62,1% for FEC and TWC respectively at the same dose for the same period of treatment. Conclusion: These results suggest the possible use of this medicinal plant in the control of gastrointestinal nematodes in sheep and justify their use in traditional veterinary practices.
References
Ademola, I.O., Fagbemi, B.O., Idowu, S.O. (2007). Anthelmintic efficacy of Nauclea latifolia extract against gastrointestinal nematodes of sheep: In vitro and In vivo studies. African. Journal of Traditional, Complementary and Alternative Medicines. 2007;4(2):148-156.
Abdullahi, R., Abubakar, Mainul Haque (2020). Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy and Bioallied Sciences ¦ Volume 12 ¦ Issue 1 ¦ January-March 2020.
Al-Shaibani, I.R.M., Phulan, M.S., Arijo, A., Qureshi, T.A. (2008). Epidemiology of Ovine Gastrointestinal Nematodes in Hyderabad District, Pakistan. Pakistan Vet. J. 2008;28(3):125-130.
Al-Shaibani, I.R.M., Phulan. M.S., Shiekh. M. (2009). Anthelmintic activity of Fumaria parviflora (Fumariaceae) against Gastrointestinal Nematodes of sheep. International Journal of Agriculture and Biology. 2009;11:431-436.
BURCREP (2010). Troisième recensement générale de la population et de l’habitat du Cameroun. Rapport de la présentation des résultats définitifs du 3ème RGPH 2010, 307.
Cringoli, G., Maurelli, M.P., Levecke, B., Bosco, A., Vercruysse, J., Utzinger, J., Rinaldi L. (2017). The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals. Nat. Protoc. 5, 503-515.
Dongmo, A.B., Beppe, J.G., Tsabang, N., Kamanyi, A. (2006). Analgesic Activities of the stem bark extract of Terminalia superba ENGL. Et Diels (Combretaceae). Pharmacology online. 2006;2:171-177. Scholar Publishing
Gillis, R.E., Gaastra, J.S., Linden, M.. Vander, Vigne., J.D. (2019). A species specific investigation into sheep and goat husbandry during the early European neolithic. Environ. Archaeol. 1–12. https://doi.org/10.1080/14614103.2019.1615214, 0.
Githiori, J.B., Athanasiadou, S., Thamsborg, S.M. (2006). Use of plants novel approaches for control of gastrointestinal helminthes in livestock with emphasis on small ruminants Vet Parasitol. 2006;139:308-320.
Inuwa, M., Atuman, Y.J., Yuguda, A., Aminu, Y.Z. (2021) Prevalence of Gastrointestinal Nematodes of Small Ruminants Slaughtered at Bauchi Central Abattoir, Bauchi, Bauchi State, Nigeria. J Vet Med Surg Vol.5 No.S2:006.
Jolie, N. N., Bohan., and Hoang, X. (2024). Oral Fenbendazole for Cancer Therapy in Humans and Animals. ANTICANCER RESEARCH 44:3725-3735 (2024).
Kamtchouing P, Kahpui SM, Djomeni Dzeufiet PD, Tedong L, Asongalem EA, Dimo T.(2006). Anti-diabetic activity of methanol/Methylene Chloride stem bark extracts of Terminalia superba and Canarium schweinfurthii on Streptozotocin- induced diabetic rats. Journal of Ethnopharmacology. 2006;104:306-309.
Martin, R. J., Puttachary, S., Buxton, S.K., Verma, S. and Robertson, A.P. (2015). The Conqueror Worm: recent advances with cholinergic anthelmintics and techniques excite research for better therapeutic drugs. Journal of Helminthology (2015) 89, 387–397.
Marcelo, B. M. (2009). Parasite control in the age of drug resistance and changing Agricultural practices. Veterinary Parasitology. 2009;163:229–234.
Masoko, P., Picard, J., Eloff, J.N. (2006). Antifungi activities of six South African Terminalia species (Combretaceae). Journal of Ethnopharmacology. 2005;99:301-308.
Mekonnen, G. (2021). A review on gastrointestinal nematodes in small ruminants. Advances in Applied Science Research, 12(7): 32. Available at:
https://www.primescholars.com/articles/a-review-on-gastrointestinal-nematodesin-smallruminants.pdf.
Michel, L. D., Maureen, L., Yannick, C. N., Jean-Eudes, D., Abdoulmoumini, M. and Timoléon, T. (2021). Breeder’s knowledge on ticks and tick-borne diseases and management strategies in Menoua division (Western Region of Cameroon). International Journal of Veterinary Sciences and Animal Husbandry 2021; 6(3): 12-21.
MINEPIA (Ministère de l’Elevage des Pêches, et des Industries Animales) (2021). Annuaire statistique du sous-secteur de l’élevage, des pêches et des industries animales. 91.
Njomen, G.B.S.N., Kamgang, R., Soua, P., Oyono, J., Njikam, N. (2009). Protective Effect of Methanol- Methylene Chloride Extract of Terminalia glaucescens Leaves on Streptozotocin-Induced Diabetes in Mice. Trop. J. Pharm. Res. 2009;8(1):19-26.
Perry, B., Randolph, T., McDermott, J. Sones, K. and Thornton, P. (2002): Investing in Animal Health Research to Alleviate Poverty. International Livestock Research Institute, Nairobi, Kenya, Pp: 148.
Pollott, G., Wilson, R.T. (2009). Sheep and Goats for Diverse Products and Profits; FAO diversification booklet; FAO: Rome, Italy, 2009; ISBN 978-92-5-106137-4.
Suhail Rashid Mir (2016). Anthelmintic activities of various plant extracts:General review. Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org. JETIR August 2016, Volume 3, Issue 8. (ISSN-2349-5162). Scholar Publishing
Tazen, F., Fonteh, F. (2013). Karambiri H. Gestion intégrée des ressources en eau dans le bassin versant du lac municipal de Dschang: connaissance et usage. Int. J Biol Chem. Sci 2013;7:840-851.
Tesfaye, T. (2021). Prevalence, species composition, and associated risk factors of small ruminant gastrointestinal nematodes in South Omo zone, South-Western Ethiopia. Journal of Advanced Veterinary and Animal Research, 8(4): 597-605. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757667/
Thienpont, D., Rochette, F., Vanparljs, O.F.J. (1986). Diagnosing Helminthiasis by Coprological Examination. Janssen Reseach Foundation, Beerse Belgium; 1986.
Wabo Poné, J., Mpoame, M., Bilong, B. C. F. (2009). In vivo evaluation of potential nematicidal properties of ethanol extract of Canthium mannii (Rubiaceac) on Heligmosomoides polygyrus parasite of rodents. Vet. Parasitol. 2009;166:103–107.
Wabo Poné, J., Billong, B. C.F., Mpoame, M. (2010). In vitro nematicidal activity of extracts of Canthium mannii (Rubiaceae), on different life- cycle stages of Heligmosomoides polygyrus (Nematoda, Heligmosomatidae). Journal of Helminthology 2010;84:156-165.
Wabo Poné, J., Kenne, T.F., Mpoame, M., Pamo, T. E., Bilong, B. C. F. ( 2011). In vitro activities of acetonic extract from leaves of three forage legumes (Calliandia calotysus, Gliricidia sepium and Leucaena diversifolia) on Haemonchus contortus. Asian Pacific Journal of Tropical Medicine. 2011;112-420.
World Bank. (2016). PROJECT INFORMATION DOCUMENT (PID) APPRAISAL STAGE Implementing Agency Environmental Category B-Partial Assessment Appraisal Review Decision (from Decision Note).
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