Analytical Methods for Determining the Peroxide Value of Hemp Seed (Cannabis Sativa L.) Oil

Authors

  • Yusuf Yildiz Best Care Nursing College, Science Department; East Orange, New Jersey, USA
  • Yvan Duchaine Best Care Nursing College, Science Department; East Orange, New Jersey, USA
  • Zehra N. Ergin Bergen County Academies, Hackensack, New Jersey, USA

DOI:

https://doi.org/10.14738/aivp.1304.19265

Keywords:

Peroxide Value, Cannabis Sativa, Hemp Seed Oil, Sodium thiosulphate

Abstract

The peroxide Value is the number that expresses, in milli equivalents of active oxygen, the quantity of peroxide contained in 1000 g of the substance. Hemp seed oil containing essential omega fatty acids and proteins. This organic hemp seed oil has a pleasant nutty smell, deep green hue, and absorbs well into the skin. Hemp-seed oil has several positive effects on skin problems such as dryness and those related to the aging process. Hemp seed oil sample were peroxide value (POV) assayed using a standard starch solution, American Oil Chemist’s Society (AOCS) Method Cd 8-53, and used as a verified reference method for peroxide determination. Peroxide values (POV) is a standard test in the food and cosmetic industry to ensure product quality and self-life. In this study, peroxide value in Hemp Seed (Cannabis Sativa L.) oil has been determined by iodometric titration method using acetic acid-chloroform reagent. The average result is less than 4.0 meq O2 / Kg. Relative repeatability Standard Deviation %RSD is 2.64 %. The average result meets the specification.

References

K. Sunil, B. Narayana. Spectrophotometric Determination of Hydrogen Peroxide in Water and Cream Samples. Bull Environment Contamination Toxicology (2008) 81: 422-426 DOI: 10.1007/s00128-008-9477-7

Handbook of Detergents, Part C: Analysis Edited by Heinrich Waldhaff, Rudiger Spilker, New York, 2004. P: 460-463.

GFS Chemicals, Inc. 245 Powel, OH 43065.

Gordon M. H. Understanding and Measuring the Self-Life of Food. Woodhead Publishing; Sawston, UK: 2004.

Gordon M. H. Antioxidants in Food. Woodhead Publishing; Sawston, UK: 2001.

Guillen M. D., Ruiz A. Monitoring the oxidation of unsaturated oils and formation of oxygenated aldehydes by proton NMR. Eur. J. Lipid Sci. Technol. 2005; 107:36-47. DOI: 10.1002/ejlt.200401056.

Gotoh N., Wada S. The Importance of Peroxide Value in Assessing Food Quality and Food Safety. J. Am. Oil Chem Soc. 2006; 83:473-474. Doi: 10.1007/s11746-006-1229-4.

American Oil Chemist’s Society. Official Methods and recommended Practices of the American Oil Chemists Society. 4th ed. AOCS Press; Champaign, IL, USA: 1998. Method Cd 8b-90.

Commission Regulation (EEC) no. 2568/91 on Characteristics of olive oil and olive residue oil and on the relevant methods of analysis. Off. J. Eur. Communities. 1991; L248:1-83.

William E. Gilbraith, J. Chance Carter, Kristl L. Adams, Karl S. Booksh, and Jashua M. Ottaway- Improving Prediction of Peroxide Value of Edible Oils Using Regularized Regression Models. Stefeno Materazzi, Academic Editor Nov 30, 2021, 26 (23): 7281 DOI: 10-3390/molecules 26237281

Center for Food Safety and Applied Nutrition. Food and Drug Administration, Washington, DC. 20204, 1999 Nov 16, p. 2:29

ISO-Standard 3960 third edition 2001 AOCS CD 8b-90 European Pharmacopeia Leatherhead Food RA, second edition

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Published

2025-08-26

How to Cite

Yildiz, Y., Duchaine, Y., & Ergin, Z. N. (2025). Analytical Methods for Determining the Peroxide Value of Hemp Seed (Cannabis Sativa L.) Oil. European Journal of Applied Sciences, 13(04), 364–369. https://doi.org/10.14738/aivp.1304.19265