Tissue Culture of Bambusa Nutans Wall ex Munro and Dendrocalamus Asper (Schult. & Schult.f.) Backer ex Heyne: Optimizing Protocol for Commercial Exploitation
DOI:
https://doi.org/10.14738/aivp.1304.19335Keywords:
B. nutans, D. asper, Bud proliferation, BAP, NAA, HardeningAbstract
Bamboo is a fast growing, highly versatile bioresource plant that significantly contributes to both industrial applications and ecological balance. The present study focuses on optimizing tissue culture protocols for commercial exploitation of two economically important bamboo species Bambusa nutans Wall ex Munro and Dendrocalamus asper (Schult. & Schult.f.) Backer ex Heyne. We employed nodal segments as explants, which were surface sterilized and grown in Murashige and Skoog (MS) medium enriched with different plant growth regulators (alone or in various combinations). Seasonal variations in bud initiation and proliferations were monitored. The experiments were conducted in triplicate using a completely randomized design with 10 replicates each and significance levels were determined through Analysis of variance (ANOVA), with mean comparison performed using Duncan’s Multiple Range Test (DMRT) at p≤0.05. The rate of bud initiation was observed during mid spring to summer. The maximum number of shoots (4.80 ± 0.28 in B. nutans and 5.04 ± 0.05 in D. asper) were achieved in MS medium containing 5.0 mg/l BAP, however, the shoot length was significantly high in 2.5mg/l of BAP. Liquid media promoted better shoot growth and quality. NAA and additives had positive influence on shoot multiplication in both the species. For root formation in both species, NAA (0.5 mg/l) in half strength of basal medium was the most effective among all the auxins.
References
Acharya, S., Ghimire, S., Adhikari, C., & Chaurel, S. (2024). Effect of growing media on germination and seedling growth of tomato varieties. Asian Journal of Agricultural and Horticultural Research, 11(1), 49–61
Ahmad, Z., Teixeira da Silva, J. A., Shahzad, A., Lin, S., Ding, Y., & Ramakrishnan, M. (2023). Biotechnological interventions in bamboo plants. Plant Cell, Tissue and Organ Culture (PCTOC), 153(3), 459-487
Anand M, Brar J, Sood A (2013) In vitro propagation of an edible bamboo Bambusa bambos and assessment of clonal fidelity through molecular markers. J Med Bioeng 2(4):257–261
Arya, I. D., & Arya, S. (1997). Micropropagation of bamboo and its application. Plant Cell, Tissue and Organ Culture, 51(2), 89–93
Arya, I. D., & Sharma, S. (1998). Micropropagation of Bambusa bambos through shoot proliferation. Plant Cell Reports, 17(6–7), 427–430.
Arya, I. D., & Sharma, S. (2009). Micropropagation protocol for Bambusa nutans Wall. ex Munro. Indian Journal of Forestry, 32(1), 37–44
Arya, I. D., Arya, S., & Sharma, S. (1999). Micropropagation of bamboo (Bambusa nutans). Plant Cell Reports,
Arya, I. D., Arya, S., & Sharma, S. (2001). Micropropagation of bamboos: Advances and future prospects. Indian Forester, 127(5), 597–612.
Arya, I. D., Kaur, B., & Arya, S. (2012). Rapid and mass propagation of economically important Bamboo Dendrocalamus hamiltonii. Indian Journal of Energy, 1(1), 11-16.
Arya, I. D., Satsangi, R., & Arya, S. (2001). Rapid micropropagation of edible bamboo Dendrocalamus asper. Journal of Sustainable Forestry, 14(2-3), 103-114.
Banerjee, A., Sarkar, D., & Saha, S. (2022). Comparative efficacy of surface sterilants in nodal cultures of woody plants. Plant Biotechnology Reports, 16(4), 381–392
Banerjee, M., Gantait, S., & Pramanik, B. R. (2011). A two step method for accelerated mass propagation of Dendrocalamus asper and their evaluation in field. Physiology and Molecular Biology of Plants, 17(4), 387-393.
Banik, R. L. (2015). Bamboo silviculture. In Bamboo: the plant and its uses (pp. 113-174). Cham: Springer International Publishing.
Bhatia, P., & Ashwath, N. (2008). Improving the quality of in vitro cultured plant tissues by addition of antioxidant compounds. Plant Cell, Tissue and Organ Culture, 92(3), 285–290.
Bhojvaid, P. P., & Timmer, V. R. (1998). Soil dynamics in an age sequence of Prosopis juliflora planted for sodic soil restoration in India. Forest Ecology and Management, 106(2-3), 181-193.
Canavan, S., Richardson, D. M., Visser, V., Le Roux, J. J., Vorontsova, M. S., & Wilson, J. R. (2017). The global distribution of bamboos: assessing correlates of introduction and invasion. AoB Plants, 9(1), plw078.
Chauhan, A., Bensaad, M. S., Pant, M., Kahoul, M. A., Mitra, D., Mohapatra, P. K. D., & Panneerselvam, P. (2024). Bioinformatics and Its Applications in Plant Tissue Culture. In Tissue Culture Techniques and Medicinal Plants (pp. 307-320). CRC Press.
Chongtham, N., & Bisht, M. S. (2020). Bamboo shoot: superfood for nutrition, health and medicine. CRC Press.
Choudhary P, Das M, Sikdar SR, Pal A (2004). Influence of the physiological age and position of the nodal explants on micropropagation of field-grown Dendrocalamus strictus Nees. Plant Cell Biotechnology and Molecular Biology 5(1-2):45
Choudhary A.K., Ranjan A., Kumari P. (2016). In vitro shoot proliferation for rapid and mass production of quality planting materials of Bambusa nutans in the climatic conditions of Bihar, India. Indian Journal of Energy 5(2):1-11.
Choudhary A.K., Kumar A., Kumari P., Kumari S., Kumar R., Ranjan R., Khan I.(2025a).Bamboo: A symbol of Tradition faith, and Climate Resilience. Applied Science Research Periodicals,3(4),192-209
Choudhary, A. K., Kumari, P., & Kumari, S. (2025b). Tissue culture of Bengal Bamboo (Bambusa tulda Roxb.). Narendra Publishing House, New Delhi. Pp, 129.
Choudhary, A. K., Kumari, P., & Kumari, S. (2022). In vitro propagation of two commercially important bamboo species (Bambusa tulda Roxb. and Dendrocalamus stocksii Munro.). African Journal of
Choudhary, A. K., Kumari, S., & Kumari, P. (2020b). Factors influencing in vitro regeneration of Bamboos (Bambusa balcooa Roxb. and Bambusa tulda Roxb. ) for rapid mass clonal propagations of high quality planting materials in agroclimatic conditions of Bihar. Indian Journal of Tropical Biodiversity. 28 (1& 2)
Choudhary, A. K., Priyanka, K., & Ashish, R. (2017). Refinement of protocol for rapid clonal regeneration of economical bamboo, Bambusa balcooa in the agroclimatic conditions of Bihar, India. African Journal of
Choudhary, A. K., Ranjan, A., Kumari, S., & Tudu, S. (2020a). In vitro culture and adventitious rhizogenesis of Bambusa tulda Roxb. in agro-climatic conditions of Bihar, India. South Asian Journal of Experimental Biology, 10(1). URL: http://dx.doi.org/10.14738/aivp.1304.19335 387
Das, M., & Pal, A. (2005). In vitro regeneration of Bambusa balcooa Roxb.: Factors affecting changes of morphogenetic competence in the axillary buds. Plant Cell, Tissue and Organ Culture, 81(1), 109–112. https://doi.org/10.1007/s11240-004-3017-x
Das, M., Pal, A., & Choudhury, D. (2019). Improvement of bamboo micropropagation efficiency by sequential sterilization. Physiology and Molecular Biology of Plants, 25(4), 977–986
Das, R., Mitra, S., & Bera, A. (2020). Auxin-induced rhizogenesis in bamboos: A comparative study of IAA and NAA in shoot-derived explants. Indian Journal of Plant Physiology, 25(4), 644–652
Devi, P., Singh, A., & Choudhury, D. (2021). Micropropagation and conservation of economically important bamboo species. Journal of Applied and Natural Science, 13(2), 478–487
Dewir, Y. H., El-Mahrouk, M. E., & Murthy, H. N. (2018). In vitro rooting of difficult-to-root plants. Horticultural Plant Journal, 4(1), 1–9.
Domínguez, J., Sanchez-Hernandez, J. C., & Lores, M. (2017). Vermicomposting of winemaking by-products. In Handbook of grape processing by-products (pp. 55-78). Academic Press.
Dutta, S., Gorain, S., Roy, J., Das, R., Banerjee, S., Gorai, S. K., ... & Das, S. (2025). Bamboo for global sustainability: A systematic review of its environmental and ecological implications, climate action, and biodiversity contributions. Environmental Reviews, (ja).
Edwards, C. A., Arancon, N. Q., Vasko-Bennett, M., Askar, A., & Keeney, G. (2010). Effect of aqueous extracts from vermicomposts on attacks by cucumber beetles (Acalymna vittatum) (Fabr.) on cucumbers and tobacco hornworm (Manduca sexta)(L.) on tomatoes. Pedobiologia, 53(2), 141-148.
Faisal, M., Alatar, A. A., & Ahmad, N. (2018). Role of amino acids in plant tissue culture: A review. Plant Cell, Tissue and Organ Culture, 132(3), 513–533.
Food and Agriculture Organization [FAO]. (2010). Global forest resources assessment 2010. Rome: FAO.
Food and Agriculture Organization [FAO]. (2022). Bamboo: A strategic resource for sustainable development. Rome: FAO.
Forest Survey of India [FSI]. (2023). India State of Forest Report 2023. Ministry of Environment, Forest and Climate Change, Government of India
Gairola, S. U., Bhuvaneswari, V., & Khanduri, A. K. (2025). Exploring bamboo’s potential in economic returns and degraded land restoration in Uttarakhand, India. Sustainability in Mountain Regions, 2(1), 1–
George, E. F., Hall, M. A., & De Klerk, G.-J. (2008). Plant propagation by tissue culture (3rd ed.). Springer.
Goyal, A. K., Pradhan, S., Basistha, B. C., & Sen, A. (2015). Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) nees using RAPD and ISSR markers. 3 Biotech, 5(4), 473-482.
Gupta, S., Khurana, P., & Sinha, R. (2019). Antioxidant-mediated improvement in bamboo micropropagation. Physiology and Molecular Biology of Plants, 25(4), 987–996.
Haque, S. M., Sarker, S., & Alam, S. (2020). Effects of auxins on in vitro propagation of bamboo. International Journal of Botany Studies, 5(5), 126–132.
Hassan, N., Abid, S., & Khan, M. A. (2022). Chelating agents in plant tissue culture: Roles and mechanisms. Plant Biotechnology Reports, 16(5), 689–701.
Hazarika, B. N. (2003). Morphogenetic response of citrus in vitro to varying sucrose level.111-116.
Hyde, K. D., Jayawardena, R. S., Mckenzie, E. H. C., Wanasinghe, D. N., Liu, Y. G., & Xiao, Q. G. (2024). Bambusicolous mycopathogens in China with an update on taxonomic diversity, novel species, pathogenicity, and new insights.
Indian Network for Bamboo and Rattan [INBAR]. (2021). Bamboo for sustainable development. Beijing:
Islam, M. A., & Bari, M. A. (2014). An efficient sterilization protocol for micropropagation of bamboo (Bambusa wamin and Dendrocalamus asper). American Journal of Plant Sciences, 5(15), 2404–2409.
Jha, K. K., Singh, A., & Sinha, A. (2022). Bamboo resources of Bihar: Distribution, uses and challenges. Indian Forester, 148(7), 621–629.
Kalaiarasi, K., Sangeetha, P., Subramaniam, S., & Venkatachalam, P. (2014). Development of an efficient protocol for plant regeneration from nodal explants of recalcitrant bamboo (Bambusa arundinacea Retz. Willd) and assessment of genetic fidelity by DNA markers. Agroforestry systems, 88(3), 527-537.
Korwar, G. R., Prasad, J., Rao, G. R., & Venkatesh, G. (2013). Agroforestry as a strategy for livelihood security in the rainfed areas: Experience and expectations. In Agroforestry – Systems and Prospects (pp. 79–96). Springer.
Kumar, A., Bargali, H., Bisht, K., & Pant, K. (2025). Role of bamboo-based agroforestry in climate change mitigation and livelihood: Indian Himalayan Region perspective. In Climate change impact on Himalayan biodiversity (pp. 479-500). Cham: Springer Nature Switzerland.
Kumar, A., Bargali, H., Bisht, K., & Pant, K. (2025). Role of bamboo-based agroforestry in climate change mitigation and livelihood: Indian Himalayan Region perspective. In Agroforestry and Climate Resilience (pp. 385–401). Springer.
Kumar, P., Singh, V., & Tiwari, R. (2021). Bamboo diversity and its conservation in India: A review. Indian Journal of Ecology, 48(2), 365–374.
Kumar, R., Singh, V., & Singh, A. (2020). Optimization of surface sterilization protocols for woody bamboos. Journal of Tropical Forestry, 36(2), 45–53.
Kumari, S. (2022). Studies on refinement of protocol for rapid and mass in vitro clonal propagation and evaluation of growth performance of Bambusa tulda Roxb. in Bihar. Ph. D Thesis, Tilkamanji Bhagalpur University, Bhagalpur.
Lestari, E. G., Fitriani, R., & Wulandari, R. A. (2023). Effect of liquid culture system on shoot multiplication and quality of banana (Musa spp.) explants. Biocell, 47(3), 713–722.
Li, C. E., Lee, S. Y., Chen, Y. Y., Kuo, S. Y., & Yuan, M. H. (2025). Bamboo ecosystem services in 25 years: a systematic literature review of trends, insights, and knowledge gaps. Environmental Science and Pollution Research, 1-14
Li, M., Rani, M., Lathwal, M., Singh, A. N., & Chongtham, N. (2023). Bamboo as a phytoremediation candidate for heavy metal–contaminated soil: A synthesis. In Bamboo Science and Technology (pp. 125–145). Springer.
Liese, W., & Kohl, M. (2015). Bamboo. The plant and its uses. Switzerland.
Lobovikov, M., Paudel, S., Piazza, M., Ren, H., & Wu, J. (2009). World bamboo resources: A thematic study. FAO, Rome.
Lobovikov, M., Schoene, D., & Yping, L. (2012). Bamboo in climate change and rural livelihoods. Mitigation and Adaptation Strategies for Global Change, 17(3), 261-276.
Lobovikov, M., Shyam, S., & Singh, A. (2019). Bamboo resources and utilization in India. INBAR Technical Report. Beijing: INBAR.
Lobovikov, M., Zhou, L., & Wu, J. (2020). Bamboo and rattan for sustainable development. Beijing: INBAR
Luo, X., Wang, X., Ren, H., Zhang, S., & Zhong, Y. (2022). Long-term mechanical properties of bamboo scrimber. Construction and Building Materials, 338, 127659.
Maina, J. W. (2023). Adoption of Sustainable Bamboo Farming to Mitigate the Effects of Soil Degradation and to Improve Livelihood in Kinale, Kiambu County, Kenya (Doctoral dissertation, Kenyatta University).
Maiya, S. M., Janardan, L., & Prasad, G. D. (2021). The impact of various factors of in vitro culture on shoot multiplication and plant production of the Bambusa nutans subsp. cupulata in in vitro propagation through nodal segments. Int J Res Anal Rev, 8, 766-776.
Maurya, L. L., Singh, Y. A., Bhardwaj, A., Kumar, V., Jamaludheen, V., & Shil, S. (2025). Diversity, Threats and Conservation Aspects of Bamboo: A Review on Green Gold. International Journal of Ecology and Environmental Sciences, 51(1), 27-35.
Mchumo, A., Wang, R. (2025). Bamboo: A Potential Tool for Climate Change Mitigation and Adaptation. In: Wang, H.H., Miao, M.L. (eds) Global Development and Cooperation with China. China and Globalization. Springer, Singapore
Mudoi, K. D., & Borthakur, M. I. N. A. (2012). Factors affecting the frequency of in vitro flowering of Bambusa balcooa Roxb. Indian J Plant Physiol, 17, 37-43.
Mudoi, K. D., Saikia, S. P., & Borthakur, M. (2014). Effect of nodal positions, seasonal variations, shoot clump and growth regulators on micropropagation of commercially important bamboo, Bambusa nutans Wall. ex. Munro. African Journal of Biotechnology, 13(19), 1961-1972.
Mudoi, K. D., Saikia, S. P., & Borthakur, M. (2014). Micropropagation of bamboos: An overview. Research Journal of Biotechnology, 9(1), 1–15.
Mudoi, K. D., Saikia, S. P., Goswami, A., Gogoi, A., Bora, D., & Borthakur, M. (2013). Micropropagation of important bamboos: a review. African Journal of Biotechnology, 12(20).
Mustafa, A. A., Derise, M. R., Yong, W. T. L., & Rodrigues, K. F. (2021). A concise review of Dendrocalamus asper and related bamboos: germplasm conservation, propagation and molecular biology. Plants, 10(9), 1897.
Nadgauda, R. S., John, C. K., & Mascarenhas, A. F. (1993). Floral biology and breeding behavior in the bamboo Dendrocalamus strictus Nees. Tree Physiology, 13(4), 401-408.
Naik, S. K., Shinde, R., Mali, S. S., Sarkar, P. K., & Das, A. (2025). Land Degradation: A Global Challenge to Environmental Sustainability and Livelihood Security. In Ecological Solutions to Agricultural Land Degradation (pp. 1-27). Singapore: Springer Nature Singapore.
National Bamboo Mission [NBM]. (2020). Bamboo development in Bihar: Status and prospects. Ministry of Agriculture & Farmers’ Welfare, Government of India.
Negi, D., & Saxena, S. (2011). Micropropagation of bamboos through in vitro techniques: A review. Plant Biotechnology Reports, 5(1), 3–22.
Negi, D., & Saxena, S. (2011). Micropropagation of Bambusa balcooa Roxb. through axillary shoot proliferation. In Vitro Cellular & Developmental Biology – Plant, 47(6), 604–610. https://doi.org/10.1007/s11627-011-9403-2
Negi, D., & Saxena, S. (2011). Season-dependent culture establishment of nodal explants from mature trees of Dendrocalamus hamiltonii. Plant Cell Reports, 30(9), 1667–1678.
Negi, M., Nautiyal, S., Pradhan, R., & Goswami, M. (2022). Exploring Wild Edibles of Male Mahadeshwara Betta (MM Hills) and their potential for socio economic development of local people: A scientific and technological interventions approach. International Journal of Ecology and Environmental Sciences, 49(1), 83-93.
Nirmala, C., Bisht, M. S., & Laishram, M. (2018). Bioactive compounds in bamboo shoots: Health benefits and prospects. Pharmacognosy Reviews, 12(23), 59–65. https://doi.org/10.4103/phrev.phrev_22_17
Pan, C., Zhou, G., Shrestha, A. K., Chen, J., Kozak, R., Li, N., Li, J., He, Y., Sheng, C., & Wang, G. (2023). Bamboo as a Nature-Based Solution (NbS) for Climate Change Mitigation: Biomass, Products, and Carbon Credits. Climate, 11(9), 175.
Pati, P. K., Sharma, M., & Ahuja, P. S. (2006). Prevention of tissue browning in callus cultures of Seabuckthorn (Hippophae rhamnoides L.) by antioxidants. Biologia Plantarum, 50(4), 565–568.
Pérez-Pazos, J., Rosero, A., Vergara, E., & Gámez, R. (2023). Response of sweet potato to substrates and acclimatization conditions in the greenhouse to produce high-quality planting material. The Horticulture Journal, 92(4), 451-463.
Purkayastha, J., Sugla, T., Paul, A., Solleti, S. K., & Sahoo, L. (2017). Rapid in vitro regeneration of a commercially important bamboo (Dendrocalamus hamiltonii). Plant Cell, Tissue and Organ Culture, 88(1), 1–8.
Rajput, D. S., Rathore, T. S., Ansari, S. A., & Faisal, M. (2023). Biochemical changes in embryogenic and nonembryogenic callus of Bambusa nutans Wall. during somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 154, 639–652
Ramesh, S., Saravanan, M., & Kumar, T. S. (2020). Control of phenolic browning in plant tissue culture: A review. Journal of Applied Biology & Biotechnology, 8(4), 53–61
Rao, I. U., Srinivas, K., & Reddy, T. P. (2017). Influence of plant growth regulators on bamboo in vitro cultures. Vegetos, 30(1), 1–8.
Rao, F., Ji, Y., Yang, Y., Zhang, Y., Li, N., Yu, W., & Chen, Y. (2021). Rapid process natural bamboo into outdoor bamboo-fiber-reinforced composite with high surface photostability. Forests, 12(4), 446.
Rathour, R., H. Kumar, K. Prasad, P. Anerao, M. Kumar, A. Kapley, A. Pandey, M. Kumar Awasthi, and L. Singh. 2022. “Multifunctional Applications of Bamboo Crop Beyond Environmental Management: An Indian Prospective.” Bioengineered 13 (4): 8893–8914.
Rout, G. R., & Das, P. (1994). Micropropagation of bamboos: A review. Plant Cell, Tissue and Organ Culture, 39(1), 1–12
ROYAL BOTANIC GARDENS, KEW (RBGK). Plants of the world online 2024.
Sawarkar, A. D., Shrimankar, D. D., & Manekar, S. C. (2025). Bamboo as a sustainable crop for land restoration in India: Challenges and opportunities. Environment, Development and Sustainability.
Seethalakshmi, K. K., & Kumar, M. S. M. (1998). Bamboos of India: A compendium. Kerala Forest Research Institute, Kerala
Sharma, M. L., Kumar, D., & Singh, K. (2023). Agroforestry potential of Dendrocalamus asper in India. Agroforestry Systems, 97(4), 551–564
Sharma, R., Bhattacharya, A., & Das, M. (2021). Comparative evaluation of sterilants in bamboo nodal explants. Plant Biotechnology Reports, 15(5), 621–631.
Sharma, V., Patel, H., & Chauhan, N. (2024). IAA-mediated root induction in monocots: Insights from in vitro culture systems. Current Trends in Plant Science, 30(2), 99–108
Shirin, F., & Rana, P. K. (2007). In vitro clonal propagation of Bambusa glaucescens. Scientia Horticulturae, 113(1), 96–101.
Silveira, A. A. D. C., Lopes, F. J. F., & Sibov, S. T. (2020). Micropropagation of Bambusa oldhamii Munro in heterotrophic, mixotrophic and photomixotrophic systems. Plant Cell, Tissue and Organ Culture (PCTOC), 141(2), 315-326.
Singh, L., Sridharan, S., Thul, S. T., Kokate, P., Kumar, P., Kumar, S., & Kumar, R. (2020). Eco-rejuvenation of degraded land by microbe assisted bamboo plantation. Industrial Crops and Products, 155, 112795.
Singh, G., & Pandey, A. (2024). Climate change induced disasters and highly vulnerable infrastructure in Uttarakhand, India: current status and way forward towards resilience and long-term sustainability. Sustainable and Resilient Infrastructure, 9(2), 145-167.
Singh, M., & Bhatnagar, P. (2023). Auxin-based root induction in Dendrocalamus asper: A step toward improved clonal propagation. Asian Journal of Plant Science & Research, 13(1), 24–30.
Singh, R., Devi, P., & Choudhury, D. (2020). Bamboo biotechnology: Advances and prospects. Trees, 34(4), 729–742 URL: http://dx.doi.org/10.14738/aivp.1304.19335 391
Singh, P., Dwivedi, R. P., & Arunachalam, A. (2023). Agroforestry solutions for zero hunger and net zero climate targets. Curr Sci, 124(6), 661-662.
Singh, S. R., Dalal, S., Singh, R., Dhawan, A. K., & Kalia, R. K. (2012). Micropropagation of Dendrocalamus asper {Schult. & Schult. F.} Backer ex k. Heyne): an exotic edible bamboo. Journal of Plant Biochemistry and
Solomon, T., Moon, H., Abebe, S., Minale, A. S., & Teketay, D. (2021). Promoting bamboo-based agroforestry for enhancing ecosystem services from degraded lands. In Agroforestry for Degraded Landscapes: Recent Advances and Emerging Challenges-Vol. 2 (pp. 423-444). Singapore: Springer Singapore.
Suwal, M. M., Lamichhane, J., & Gauchan, D. P. (2020). Regeneration technique of bamboo species through nodal segments: A review. Nepal Journal of Biotechnology, 8(1), 32–42
Suwal, M. M., Lamichhane, J., & Gauchan, D. P. (2021). Assessment of genetic stability of micropropagated Bambusa balcooa Roxb. using RAPD marker. Plant Tissue Culture and Biotechnology, 31(1), 57–65.
Trivedi, A., Bhati, S., & Joshi, A. (2024). Establishment of callus cultures and quantification of Caffeic acid using HPTLC from Desmodium gangeticum (L.). Natural Product Research, 1-8.
Wei, Q., Cao, J., Qian, W., Xu, M., Li, Z., & Ding, Y. (2015). Establishment of an efficient micropropagation and callus regeneration system from the axillary buds of Bambusa ventricosa. Plant Cell, Tissue and Organ Culture (PCTOC), 122(1), 1-8.
Widjaja, E. A., Sumarna, Y., & Astuti, R. (2023). Taxonomy and utilization of Bambusa nutans and related species. Reinwardtia, 22(1), 23–36.
Yang, X., Li, Y., & Chen, H. (2022). Bamboo as a carbon sink: Potential and challenges. Forests, 13(8), 1286 ZANI, W. N. W. M., SIDIK, N. J., ISA, N. A. M., AWAL, A., OSMAN, N. I., ALIAS, N., & NORDIN, M. K. (2024). Influence of media variations and growth regulators on in vitro propagation of Dendrocalamus asper bamboo. Nusantara Bioscience, 16(2).
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