Spectroscopic Investigation of the Neuroprotective Effect of Lepidium sativum Water Extract Against 2, 4-Dichlorophenoxyacetic Acid Toxicity on Rat Brain Cerebellum Tissue
DOI:
https://doi.org/10.14738/aivp.1303.18982Keywords:
Fourier transform infrared spectroscopy (FTIR), Antioxidants, Neurotoxicity, Membrane, Protein, herbicide, FT-IR: Fourier Transform Infrared, LS: Lepidium sativum, 2, 4-D: 2, 4-Dichlorophenoxyacetic acid, LD50: Half lethal dose, LDS: Least square difference, HBW: Half band width, ν: vibrational mode, νas: Asymmetric vibrational modeAbstract
FT-IR spectroscopy is a crucial analytical technique used to study complex structures of biological samples. In the present study, FTIR spectroscopy was applied to verify the neuroprotective effect of Lepidium sativum (LS) water extract against the toxicity of 2, 4-Dichlorophenoxyaceticacid in the cerebellar tissue of rat brains. Treated rats were split into four groups; water control group (water with oral gavage), protective group (LS for 4 weeks then a single dose of 2, 4-D LD50), curative group (a single dose of 2, 4-D LD50 then LS for 4 weeks) and LS only group (LS for 4 weeks), a minimum of 5 rats in each group. The group treated with LS alone exhibited a clear recovery from the significant decrease in the protein content after 2, 4-D intoxication, expressed at the amide I band centered at ~1645cm-1. Additionally, significant improvements were observed in the alterations of the secondary protein structure in 2, 4-D intoxicated groups treated with LS extract. Furthermore, notable changes were detected in the looseness of membrane lipid chain packing, lipid polarity and/or a significant rise in the formation of lipids hydroperoxyl groups and carbonyl compounds. However, this effect in the protective group was not as significant as the curative and LS only groups. To conclude, this study demonstrates that LS water extract has a significant potency as an antitoxic/antioxidant effect against neurotoxicity induced by 2, 4-D. Moreover, FT-IR spectroscopy has proven to be rapid and sensitive in toxicity monitoring as well as treatment in biological membranes.
References
El-Gendy, M., El-Gezawy, E., Saleh, A., Alhotan, R., Al-Badwi, M., Hussein, E., El-Tahan, H., Kim, I., Cho, S., Omar, S., 2023. Investigating the Chemical Composition of Lepidium sativum Seeds and Their Ability to Safeguard against Monosodium Glutamate-Induced Hepatic Dysfunction. Foods. 12, 4129.
Singh, B., Lubhani, Monzur, M., Sri, M., Ashoor, Kaur, J., Singh, J., 2022. Lepidium sativum: Its nutritional composition and therapeutic properties. The Pharma Innovation Journal. 11(7): 15-25
Gupta, S., Gupta, R., 2024. Research Update on the Therapeutic Potential of Garden Cress (Lepidium sativum Linn.) with Threatened Status. Curr Drug Res Rev.16(3) 369 – 380.
Pinto, T., Guitarte, J., Dias, M., Montagner, C., Espindola, E., González, A., 2024. New insights about the toxicity of 2,4-D: Gene expression analysis reveals modulation on several subcellular responses in Chironomus riparius. Pesticide Biochemistry and Physiology, 204 106088.
Asraoui, F., Kounnoun, A., El Cadi, H., Cacciola, F., El Majdoub, Y., Alibrando, F., Mandolfino, F., Dugo, P., Mondello, L., Louajri, A., 2021. Phytochemical Investigation and Antioxidant Activity of Globularia alypum L. Molecules. 26, 759-771.
Chatoui, K., Harhar, H., El Kamli, T., Tabyaoui, M., 2020. Chemical Composition and Antioxidant Capacity of Lepidium sativum Seeds from Four Regions of Morocco. Evidence-Based Complementary and Alternative Medicine. 4, 1-7.
Corradi, V., Besian I. Sejdiu, Haydee Mesa-Galloso, Haleh Abdizadeh, Sergei Yu. Noskov, Siewert J. Marrink, and D. Peter Tieleman, 2019. Emerging Diversity in Lipid−Protein Interactions. Chemical Reviews. 119 (9), 5775-5848 Nadkarni′S, K.M., 1995. A focus of tissue necrosis increases renal. Indian materica medica. 1, 736-744.
Dixit, J., Iii, V., Kumar, I., Palandurkar, K., Giri, R., and Giri, K., 2020. Lepidium sativum: Bone healer in traditional medicine, an experimental validation study in rats. Journal of family medicine and primary care. 9(2), 812–818.
Tahraoui, A., El Hilaly, J., Israili, Z.H., Lyoussi, B., 2007. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern morocco (errachidia province). Journal of Ethnopharmacology. 110, 105–117.
Mahassni, S.H., Al-Reemi, R.M., 2013. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (Lepidium sativum) seeds. Saudi Journal of Biological Sciences. 20(2), 131-9.
Yen, W.J., Chang, L.W., Duh, P.D., 2005. Antioxidant activity of peanut seed testa and its antioxidative component, ethyl protocatechuate, Lebensm. Wiss. Technol. 38, 193–200.
Dakhakhni, T.H., Raouf, G.A., Qusti SY., 2020a. Investigation of 2, 4-D Hepatotoxicity and Lepidium sativum Antitoxicity on Rat Tissue using FT-IR Spectroscopy. International Journal of Animal Biotechnology and Applications. 6(1), 17–29.
Kaioumova, D., Süsal, C., Opelz, G., 2001. Induction of apoptosis in human lymphocytes by the herbicide 2, 4-dichlorophenoxyacetic acid. Human Immunology. 62, 64-74.
Tayeb, W., Nakbi, A., Trabelsi, M., Attia, N., Miled, A., Hammamia, M., 2010. Hepatotoxicity induced by sub-acute exposure of rats to 2, 4-dichlorophenoxyacetic acid-based herbicide “désormone lourd”. Journal of Hazardous Materials. 180, 225-33.
Parizi, J.L.S., Tolardo, A.J., Lisboa, A.C.G., 2020. Evaluation of buccal damage associated with acute inhalation exposure to 2, 4-dichlorophenoxyacetic acid (2, 4-D) in mice. BMC Vet Res. 16, 244.
Viriato, C., França, F.M., Santos, D.S., Marcantonio, A.S., Badaró-Pedroso, C., Ferreira, C.M., Evaluation of the potential teratogenic and toxic effect of the herbicide 2, 4-D (DMA® 806) in bullfrog embryos and tadpoles (Lithobates catesbeianus). Chemosphere. 266, 129-135. Ueda, R.M.R., de Souza, V.M., Magalhães, L.R., Chagas, P.H.N., Veras, A.S.C., Teixeira, G.R., Nai, G.A., 2021. Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2, 4-D) - a simulation of environmental exposure in adult rats. J Environ Sci. Health B. 1-11.
DeQuattro, Z.A., Karasov, W.H., 2016. Impacts of 2, 4-dichlorophenoxyacetic acid aquatic herbicide formulations on reproduction and development of the fathead minnow (Pimephales promelas). Environmental Toxicology. 35(6), 1478-1488
Shafeeq, S., Mahboob, T., 2021. 2, 4-Dichlorophenoxyacetic acid induced hepatic and renal toxicological perturbations in rat model: Attenuation by selenium supplementation. Toxicol Ind Health. 37(3), 152-163.
Dakhakhni, T.H., Raouf, G.A., Awatef, A., 2020b. Electron Microscopic Study of Hepatotoxicity Induced by 2, 4-Dichlorophenoxyacetic Acid in Rats. International Journal of Cell Biology and Cellular Processes. 6(2), 18–22.
Zafra-Lemos, L., Cusioli, L.F., Bergamasco, R., Borin-Carvalho, L.A., Portela-Castro, A., 2021. Evaluation of the genotoxic and cytotoxic effects of exposure to the herbicide 2,4-dichlorophenoxyacetic acid in Astyanax lacustris (Pisces, Characidae) and the potential for its removal from contaminated water using a biosorbent. Mutat Res. 865, 503-520.
U.S. EPA, 2005. Reregistration Eligibility Decision for 2, 4-D, NewYork: U.S.
Eddouks, M., Maghrani, M., Zeggwagh, N.A., Michel, J.B., 2005. Study of the hypoglycaemic activity of lepidium sativum l. Aqueous extract in normal and diabetic rats. J. Ethano. Pharmacol. 97, 391–395.
Akkas, S.B., Severcan, M., Yilmaz, O., Severcan, F., 2007a. Effects of lipoic acid supplementation on rat brain tissue: An ftir spectroscopic and neural network stud. Food Chemistry. 105, 1281–1288.
Palaniappan, P., Vijayasundaram, V., 2009. The FTIR study of the brain tissue of labeo rohita due to arsenic intoxication. Microchemical J. 91, 118-124.
Balan, V., Cosmin-Teodor, M., Florina-Daniela, C., Cristina-Mariana, U., Gianina, D., Doru, B., Ioannis, G., 2019. Vibrational Spectroscopy Fingerprinting in Medicine: from Molecular to Clinical Practice. Materials. 12, 2884 –2825.
Van de Voort, F.R., Ismail, A.A., Sedman, J., Emo, G., 1994. Monitoring the oxidation of edible oils by fourier transform infrared spectroscopy. J Am Oil Chem Soc. 71, 243–253.
Rodriguez-Casado, J., Alvarez, M.I., De Miguel, E., Toledano, A., Carmona, P., 2007. Amphetamine effects on oxidative stress and formation of proteic beta-sheet structures as revealed by ftir microspectroscopy. Biopolymers. 86, 437-446.
Shinall, H., Song, E.S., Hersh, L.B., 2005. Susceptibility of amyloid beta peptide degrading enzymes to oxidative damage: A potential alzheimer's disease spiral. Biochemistry. 44, 15345–15350.
Singh, A, Kukreti, R., Saso, L., Kukreti, S., 2019. Oxidative Stress: A Key Modulator in Neurodegenerative Diseases. Molecules. 24(8), 1583. https://doi.org/10.3390/molecules24081583
Bukowska, B., Rychlik, B., Krokosz, A., Michalowicz, J., 2008. Phenoxyherbicides induce production of free radicals in human erythrocytes: Oxidation of dichlorofluorescine and dihydrorhodamine by 2, 4-d-na and mcpana. Food Chem. Toxicol. 46, 359–367.
Kim, Y., Rose, C.A., Liu, Y., Ozaki, Y., Datta, G., Ut, A.T., 1994. FTIR and near-infrared ft-raman studies of the secondary structure of insulinotropin in the solid state: Α-helix to β-sheet conversion by phenol and/or high shear force. J. Pharm Sci. 83, 1175–1180.
Raouf, G.A., Qusti, S.Y., Ali, A.M. Dakhakhni, T.H., 2012. The mechanism of 2, 4-dichlorophenoxyacetic acid neurotoxicity on rat brain tissue by using FTIR spectroscopy. Life Science Journal, 9(4), 1686-97.
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